Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Serratia proteamaculans |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | the fluorescence intensity in 50% glycerol does not change significantly following heat treatment. The maximum fluorescence wavelength also shifts slightly, 337-340 nm and 339-340 nm | Serratia proteamaculans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia proteamaculans | B3VI58 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
72.6 | - |
pH 8.0, temperature not specified in the publication | Serratia proteamaculans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Nalpha-benzoyl-DL-Arg-4-nitroanilide + H2O | - |
Serratia proteamaculans | Nalpha-benzoyl-DL-Arg + 4-nitroaniline | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Serratia proteamaculans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
about 60% of maximum activity | Serratia proteamaculans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
fluorescence intensity of enzyme decreases with increasing heat treatment time and the maximum fluorescence wavelength shifts slightly towards longer wavelength, 340-345 nm and 342-346 nm. The fluorescence intensity in 50% glycerol does not change significantly following heat treatment. The maximum fluorescence wavelength also shifts slightly, 337-340 nm and 339-340 nm | Serratia proteamaculans |