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Literature summary for 3.4.21.83 extracted from

  • Yan, J.B.; Wang, G.Q.; Du, P.; Zhu, D.X.; Wang, M.W.; Jiang, X.Y.
    High-level expression and purification of Escherichia coli oligopeptidase B (2006), Protein Expr. Purif., 47, 645-650.
    View publication on PubMed
Search for more literature for 3.4.21.83

Cloned(Commentary)

Cloned (Comment) Organism
fusion expression of oligopeptidase B with an N-terminal histidine tag using pET28a as the expression vector. Although most of the recombinant OpdB is produced as inclusion bodies, the solubility of the recombinant protease increases significantly when expression temperature shifts from 37°C to 30°C Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester IC50: about 0.01 mM. Oligopeptidase B is much more sensitive than oligopeptidase B Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli ?
-
 ?
Nalpha-benzoyl-Arg-7-amido-4-methylcoumarin + H2O 68% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide Escherichia coli ?
-
 ?
tert-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O specific substrate for Escherichia coli oligopeptidase B, 20% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
OpdB
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 15 min, stable below Escherichia coli
50
-
 15 min, complete inactivation Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01
-
 IC50: about 0.01 mM. Oligopeptidase B is much more sensitive than oligopeptidase B Escherichia coli trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester