EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   2.7.1.77 | -999 |
- |
- |
642002 |
| 2.7.1.77 | -999 |
- |
addition of aliphatic alcohols, 0.2 M butanol, propanol or ethanol or urea induces a much more rapid inactivation at 37°C of associated forms |
642000 |
| 2.7.1.77 | -999 |
- |
probably the free apoenzyme is converted by means of cooperative interactions between regulatory sites into an enzyme-nucleotide complex which is particularly stable at 37°C |
641999 |
| 2.7.1.77 | -999 |
- |
thermal stability is reduced when metal is removed but can be restored by addition of Mg2+ |
641996 |
| 2.7.1.77 | -999 |
- |
unstable to heat, protected from inactivation by a large number of nucleotides |
641999, 642000 |
| 2.7.1.77 | 30 |
- |
pH 5.0, 30 min, stable |
642002 |
| 2.7.1.77 | 50 |
- |
5 min, stable |
641993 |
| 2.7.1.77 | 63 |
- |
pH 8.0, 3 min, 50 mM Tris-HCl buffer, 50% loss of activity |
642001 |
| 2.7.1.77 | 68 |
- |
pH 8.0, 3 min, 50 mM Tris-HCl buffer containing 10% glycerol, 50% loss of activity |
642001 |
| 2.7.1.77 | 72 |
- |
pH 8.0, 3 min, 50 mM potassium phosphate buffer containing 10% glycerol, pH 6.8, 50% loss of activity |
642001 |
