BRENDA - Enzyme Database
show all sequences of 2.7.1.77

Nucleoside phosphotransferase from Erwinia herbicola, a new membrane-bound enzyme

Chao, H.M.; J. Biol. Chem. 251, 2330-2333 (1976)

Data extracted from this reference:

General Stability
General Stability
Organism
does not lose acttivity on lyophilization
Pantoea agglomerans
not stable to SDS
Pantoea agglomerans
stable in presence of 2 M urea, but not 4 M urea
Pantoea agglomerans
the enzyme can be isolated and handled only in the presence of detergents
Pantoea agglomerans
tolerates repeated freezing and thawing
Pantoea agglomerans
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Pantoea agglomerans
Co2+
-
Pantoea agglomerans
Cu2+
-
Pantoea agglomerans
EDTA
1 mM, 53% inhibition of transferase activity
Pantoea agglomerans
Zn2+
-
Pantoea agglomerans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound to
Pantoea agglomerans
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
10 mM MgCl2, 20% enhancement of transferase activity with p-nitrophenol as the donor, no absolute requirement for metal ion
Pantoea agglomerans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
the purified enzyme shows two separable bands in SDS-PAGE: 70000 Da and 24000 Da
Pantoea agglomerans
68000
-
sucrose density gradient sedimentation
Pantoea agglomerans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pantoea agglomerans
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pantoea agglomerans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
12
-
-
Pantoea agglomerans
Storage Stability
Storage Stability
Organism
4°C, 10% glycerol, pH 7.4, stable for at least 2 months
Pantoea agglomerans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-CMP + thymidine
20% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
cytosine + TMP
-
-
-
?
3'-dAMP + thymidine
56% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + 2'-deoxyadenosine
-
-
-
?
3'-TMP + thymidine
19% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
thymidine + TMP
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
641993
Pantoea agglomerans
5'-dAMP + 3'-dAMP + nitrophenol
5'- and 3'-nucleotide products in the approximate ratio of 2:1
641993
Pantoea agglomerans
?
4-nitrophenyl phosphate + adenosine
-
641993
Pantoea agglomerans
5'-AMP + 4-nitrophenol
-
641993
Pantoea agglomerans
?
4-nitrophenyl phosphate + thymidine
24% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
4-nitrophenol + TMP
-
-
-
?
5'-AMP + thymidine
72% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
adenosine + TMP
-
-
-
?
5'-CMP + thymidine
92% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
cytidine + TMP
-
-
-
?
5'-dAMP + thymidine
-
641993
Pantoea agglomerans
5'-deoxyadenosine + TMP
-
-
-
?
5'-dCMP + thymidine
46% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
2'-deoxycytidine + TMP
-
-
-
?
5'-dGMP + thymidine
65% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + 2'-deoxyguanosine
-
-
-
?
5'-dTMP + thymidine
-
641993
Pantoea agglomerans
5'-deoxythymidine + TMP
-
-
-
?
5'-GMP + thymidine
61% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + guanosine
-
-
-
?
adenosine 3',5'-bisphosphate + thymidine
30% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
adenosine 3'-phosphate + TMP
-
-
-
?
ADP + thymidine
50% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + AMP
-
-
-
?
ATP + thymidine
18% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
ADP + TMP
-
-
-
?
D-ribose 5-phosphate + thymidine
13% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
D-ribose + TMP
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
5 min, stable
Pantoea agglomerans
General Stability (protein specific)
General Stability
Organism
does not lose acttivity on lyophilization
Pantoea agglomerans
not stable to SDS
Pantoea agglomerans
stable in presence of 2 M urea, but not 4 M urea
Pantoea agglomerans
the enzyme can be isolated and handled only in the presence of detergents
Pantoea agglomerans
tolerates repeated freezing and thawing
Pantoea agglomerans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Pantoea agglomerans
Co2+
-
Pantoea agglomerans
Cu2+
-
Pantoea agglomerans
EDTA
1 mM, 53% inhibition of transferase activity
Pantoea agglomerans
Zn2+
-
Pantoea agglomerans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound to
Pantoea agglomerans
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
10 mM MgCl2, 20% enhancement of transferase activity with p-nitrophenol as the donor, no absolute requirement for metal ion
Pantoea agglomerans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
the purified enzyme shows two separable bands in SDS-PAGE: 70000 Da and 24000 Da
Pantoea agglomerans
68000
-
sucrose density gradient sedimentation
Pantoea agglomerans
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pantoea agglomerans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
12
-
-
Pantoea agglomerans
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 10% glycerol, pH 7.4, stable for at least 2 months
Pantoea agglomerans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-CMP + thymidine
20% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
cytosine + TMP
-
-
-
?
3'-dAMP + thymidine
56% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + 2'-deoxyadenosine
-
-
-
?
3'-TMP + thymidine
19% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
thymidine + TMP
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
641993
Pantoea agglomerans
5'-dAMP + 3'-dAMP + nitrophenol
5'- and 3'-nucleotide products in the approximate ratio of 2:1
641993
Pantoea agglomerans
?
4-nitrophenyl phosphate + adenosine
-
641993
Pantoea agglomerans
5'-AMP + 4-nitrophenol
-
641993
Pantoea agglomerans
?
4-nitrophenyl phosphate + thymidine
24% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
4-nitrophenol + TMP
-
-
-
?
5'-AMP + thymidine
72% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
adenosine + TMP
-
-
-
?
5'-CMP + thymidine
92% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
cytidine + TMP
-
-
-
?
5'-dAMP + thymidine
-
641993
Pantoea agglomerans
5'-deoxyadenosine + TMP
-
-
-
?
5'-dCMP + thymidine
46% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
2'-deoxycytidine + TMP
-
-
-
?
5'-dGMP + thymidine
65% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + 2'-deoxyguanosine
-
-
-
?
5'-dTMP + thymidine
-
641993
Pantoea agglomerans
5'-deoxythymidine + TMP
-
-
-
?
5'-GMP + thymidine
61% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + guanosine
-
-
-
?
adenosine 3',5'-bisphosphate + thymidine
30% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
adenosine 3'-phosphate + TMP
-
-
-
?
ADP + thymidine
50% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
TMP + AMP
-
-
-
?
ATP + thymidine
18% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
ADP + TMP
-
-
-
?
D-ribose 5-phosphate + thymidine
13% of the activity with 5'-dAMP or 5'-dTMP
641993
Pantoea agglomerans
D-ribose + TMP
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
5 min, stable
Pantoea agglomerans
Other publictions for EC 2.7.1.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
672755
Birichevskaya
A comparison of enzymatic phos ...
Pantoea agglomerans
Biotechnol. Lett.
29
585-591
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
7
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
1
-
-
7
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658328
Barai
An improved method for the enz ...
Pantoea agglomerans
Biotechnol. Lett.
26
1847-1850
2004
-
1
-
-
-
-
-
-
-
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642002
Asano
A novel selective nucleoside p ...
Morganella morganii, Morganella morganii NCIMB10466
J. Biosci. Bioeng.
87
732-738
1999
-
-
-
1
-
-
7
2
-
-
2
-
-
2
-
-
1
-
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
-
1
-
-
-
7
-
2
-
-
2
-
-
-
-
1
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
642003
Shin
-
Characterization of nucleoside ...
Leishmania tropica
Nucleosides Nucleotides
14
13-22
1995
-
-
-
-
-
-
-
13
2
-
1
-
-
1
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
2
-
1
-
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641995
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
267-278
1986
-
-
-
-
-
-
-
8
-
-
2
-
-
3
-
1
1
-
-
2
1
-
28
1
-
-
-
8
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
8
-
-
2
-
-
-
1
1
-
2
1
-
28
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
641996
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
291-300
1986
6
-
-
-
-
1
3
-
-
6
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
6
-
-
-
-
-
1
-
3
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
642000
Tesoriere
The purification and propertie ...
Gallus gallus
Biochim. Biophys. Acta
786
231-244
1984
-
-
-
-
-
-
1
-
-
3
-
-
-
2
-
-
1
-
-
3
1
-
1
1
-
-
1
-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
1
-
3
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
641994
Prasher
Nucleoside phosphotransferase ...
Hordeum vulgare
J. Biol. Chem.
257
4931-4939
1982
-
-
-
-
-
1
6
7
-
3
2
-
-
2
-
1
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
7
-
3
2
-
-
-
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
641999
Tesoriere
Kinetic properties of a nucleo ...
Gallus gallus
Biochim. Biophys. Acta
660
348-358
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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2
-
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1
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-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
642001
Guranowski
Nucleoside phosphotransferase ...
Lupinus luteus
Biochim. Biophys. Acta
569
13-22
1979
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
641991
Brunngraber
Nucleoside phosphotransferase ...
Daucus carota
Methods Enzymol.
51
387-394
1978
1
-
-
-
-
-
11
2
-
-
1
-
-
2
-
-
1
-
-
1
1
1
10
-
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
-
-
-
-
11
-
2
-
-
1
-
-
-
-
1
-
1
1
1
10
-
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641997
Yuyama
Identification of true thymidi ...
Tetrahymena pyriformis, Tetrahymena pyriformis ST
Arch. Biochem. Biophys.
188
64-69
1978
-
-
-
-
-
-
-
-
1
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2
-
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-
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-
2
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1
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641993
Chao
Nucleoside phosphotransferase ...
Pantoea agglomerans
J. Biol. Chem.
251
2330-2333
1976
-
-
-
-
-
5
5
-
2
1
2
-
-
2
-
-
1
-
-
-
1
1
17
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
5
-
-
2
1
2
-
-
-
-
1
-
-
1
1
17
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
641138
Kit
Characterization of nucleoside ...
Gallus gallus
Arch. Biochem. Biophys.
169
66-76
1975
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
2
1
-
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-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
-
-
-
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-
-
3
-
-
-
-
-
-
-
-
2
1
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-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
641992
Rodgers
Nucleoside phosphotransferase ...
Daucus carota
J. Biol. Chem.
247
5448-5455
1972
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3
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-
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1
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-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
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1
-
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1
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-
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1
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1
3
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641990
Brunngraber
Purification and properties of ...
Daucus carota
J. Biol. Chem.
242
4834-4840
1967
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2
8
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3
1
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2
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1
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1
1
2
16
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-
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1
1
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2
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8
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3
1
-
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1
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1
1
2
16
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1
1
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