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Literature summary for 2.7.1.77 extracted from
- Nucleoside phosphotransferase from malt sprouts. III. Studies on metal ion requirement, solvent effects, kinetics and reaction mechanism (1986), Biol. Chem. Hoppe-Seyler, 367, 291-300.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetone | 25%, 2.45fold stimulation | Hordeum vulgare |  |
| acetonitrile | 8%, 2.4fold stimulation | Hordeum vulgare | |
| Dimethyl formamide | 5%, 1.8fold, stimulation | Hordeum vulgare | |
| dimethyl sulfoxide | 20%, 1.8fold stimulation | Hordeum vulgare | |
| glycerol | 60%, 1.7fold stimulation | Hordeum vulgare | |
| methanol | 30%, 2.3fold stimulation | Hordeum vulgare | |
General Stability
| General Stability | Organism |
|---|---|
| storage in 20% acetone for 1 week without loss of activity | Hordeum vulgare |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | strong competitive inhibitor | Hordeum vulgare | |
| cAMP | strong competitive inhibitor | Hordeum vulgare | |
| EDTA | cAMP and arsenate can reduce the rate of inactivation | Hordeum vulgare | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can restore activity of the metal-free apoenzyme | Hordeum vulgare | |
| Cu2+ | can restore activity of the metal-free apoenzyme to a minor extent | Hordeum vulgare | |
| Fe2+ | can restore activity of the metal-free apoenzyme to a minor extent | Hordeum vulgare | |
| Mg2+ | contains one Mg2+ per dimeric enzyme molecule, the metal-free apoenzyme is inactive | Hordeum vulgare | |
| Ni2+ | can restore activity of the metal-free apoenzyme to a minor extent | Hordeum vulgare | |
| Zn2+ | can restore activity of the metal-free apoenzyme to a minor extent | Hordeum vulgare | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sprout | - |
Hordeum vulgare | - |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal stability is reduced when metal is removed but can be restored by addition of Mg2+ | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Hordeum vulgare |
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