EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.4.3.3 | -999 |
- |
thermal inactivation of D-amino acid oxidase from Trigonopsis variabilis occurs via three parallel paths of irreversible denaturation |
672709 |
| 1.4.3.3 | 4 |
- |
24 h, 50% loss of activity |
391847 |
| 1.4.3.3 | 15 |
40 |
the response to D-serine increases with temperature, from 15 to 40°C, the sensitivity at 37°C is 75% higher than that at 25°C |
684417 |
| 1.4.3.3 | 25 |
50 |
added FAD does not stabilize DAO at and below 35°C, but it contributes up to 3.6fold extra stability to the enzyme activity at temperatures higher than 35°C, with a half-life of 60 h encapsulated DAO is 720fold more stable than the free enzyme under conditions of bubble aeration at 25°C, the soluble oxidase is stabilized by added FAD only at temperatures of 35°C or greater, at 50°C encapsulated preparations of the oxidase are much more stable than the free enzyme whose half-life is only 40 min |
685713 |
| 1.4.3.3 | 25 |
65 |
the activity profiles of soluble and immobilized DAOs at 25-65°C are similar, immobilized DAO exhibits a melting temperature of 60°C, which is 15°C higher than those for the soluble counterpart |
685758 |
| 1.4.3.3 | 25 |
65 |
the activity profiles of soluble and immobilized DAOs at 25-65°C are similar, immobilized DAO exhibits a melting temperature of 60°C, which is 8°C higher than those for the soluble counterpart |
685758 |
| 1.4.3.3 | 30 |
- |
after 30min incubation at 45°C, the activity is completely lost |
684631 |
| 1.4.3.3 | 30 |
- |
increasing the temperature to 30°C does not affect the specific activity but enhances the volumetric enzyme activity by 22% due to its effect on cell growth |
671491 |
| 1.4.3.3 | 30 |
45 |
loss of secondary structure and inactivation occurs over a range of 30°C to 45°C, the melting temperature is at 41.4°C |
685355 |
| 1.4.3.3 | 30 |
50 |
does not lose activity at temperatures up to 30°C for at least 2 h and has a half-life of 21 min at 40°C and of 2 min at 50°C when it is incubated in 100 mM TEA-HCl buffer (pH 7.6) |
684598 |
