Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.62 | - |
D-Ser | apparent value, immobilized enzyme | Rhodotorula toruloides | |
13.75 | - |
D-Ser | apparent value, free enzyme | Rhodotorula toruloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodotorula toruloides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Storage Stability | Organism |
---|---|
-20°C, immobilized on microbiosensor, more than 160 days, 2% loss of activity | Sus scrofa |
-20°C, immobilized on microbiosensor, more than 160 days, 2% loss of activity | Rhodotorula toruloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala + H2O + O2 | 104% activity compared to D-Ser | Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
D-Ala + H2O + O2 | 165% activity compared to D-Ser | Sus scrofa | pyruvate + NH3 + H2O2 | - |
? | |
D-Asp + H2O + O2 | 38.6% activity compared to D-Ser | Sus scrofa | oxaloacetate + NH3 + H2O2 | - |
? | |
D-Asp + H2O + O2 | 5.5% activity compared to D-Ser | Rhodotorula toruloides | oxaloacetate + NH3 + H2O2 | - |
? | |
D-Ser + H2O + O2 | 100% activity | Sus scrofa | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
D-Ser + H2O + O2 | 100% activity | Rhodotorula toruloides | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-amino acid oxidase | - |
Sus scrofa |
D-amino acid oxidase | - |
Rhodotorula toruloides |
DAAO | - |
Sus scrofa |
DAAO | - |
Rhodotorula toruloides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 40 | the response to D-serine increases with temperature, from 15 to 40°C, the sensitivity at 37°C is 75% higher than that at 25°C | Rhodotorula toruloides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | immobilized enzyme | Rhodotorula toruloides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.4 | 8.5 | the response to 0.01 mM D-serine increases from pH 5.4 to 8.0, reaches a maximum at pH 8.0-8.5, and decreases at higher values, the response at physiological pH (7.4) is 80.7% of the maximum response observed at pH 8.5 | Rhodotorula toruloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Sus scrofa | |
FAD | - |
Rhodotorula toruloides |