EC Number   |
Reference |
|---|
   3.4.23.B8 | - |
647848, 647861 |
| 3.4.23.B8 | amplification of a HTLV-1 protease gene fragment by polymerase chain reaction and cloning it into a pUC plasmid vector. The protease gene fragment contains an open reading frame capable of encoding the active HTLV-1 protease. To express a fusion protein of beta-galactosidase linked with the HTLV-1 protease in Escherichia coli, a plasmid DNA is constructed by insering the HTLV-1 protease gen DNA into a procaryotic expression vector, pUEX-2, consisting of a lacZ gene directed by a lambda phage Pr promoter and designated pUEX-pro. The fusion protein is successfully expressed |
647857 |
| 3.4.23.B8 | construction of a pET19-based expression clone containing HTLV-1 protease fused to a decahistidine-containing leader peptide, expression in Escherichia coli, autoprocessing of the purified fusion protein yields a 14000 Da protease |
647858 |
| 3.4.23.B8 | expressed in Escherichia coli Rosetta2(DE3)pLysS cells |
712764 |
| 3.4.23.B8 | expression of mutant L40I which prevents autolysis and enhances enzyme stability and substrate cleavage, in Escherichia coli |
718025 |
| 3.4.23.B8 | expression of truncated enzyme forms in Escherichia coli |
647852 |
| 3.4.23.B8 | expression of wild-type and mutant enzyme in Escherichia coli strain BL21(DE3) |
667390 |
| 3.4.23.B8 | expression of wild-type and mutant enzymes as maltose-binding protein fusion proteins |
669236 |
