Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.B8 extracted from

  • Kumada, H.O.; Nguyen, J.T.; Kakizawa, T.; Hidaka, K.; Kimura, T.; Hayashi, Y.; Kiso, Y.
    Development of [Ile40]HTLV-I protease inhibition assay using novel fluorogenic and chromogenic substrate (2011), J. Pept. Sci., 17, 569-575.
    View publication on PubMed
Search for more literature for 3.4.23.B8

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant L40I which prevents autolysis and enhances enzyme stability and substrate cleavage, in Escherichia coli Human T-cell leukemia virus type I

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.31
-
 N6-(7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Ala-L-Pro-L-Gln-L-Val-L-Leu-4-nitro-L-phenylalanine-L-Val-L-Met-L-His-L-Pro-L-Leu mutant L40I, pH 5.3, 37°C Human T-cell leukemia virus type I
54.7
-
 N6-(7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Ala-L-Pro-L-Gln-L-Val-L-Leu-4-nitro-L-phenylalanine-L-Val-L-Met-L-His-L-Pro-L-Leu His-tagged recombinant protein, pH 5.3, 37°C Human T-cell leukemia virus type I

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
 x * 13000, SDS-PAGE of recombinant enzyme Human T-cell leukemia virus type I

Organism

Organism UniProt Comment Textmining
Human T-cell leukemia virus type I
-
-
-

Storage Stability

Storage Stability Organism
mutant L40I does not form aggregates and is stable as a 240 microM pH 7.0 stock solution for at least 2 months at 4°C, even with freeze-thawing Human T-cell leukemia virus type I

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-(7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Ala-L-Pro-L-Gln-L-Val-L-Leu-4-nitro-L-phenylalanine-L-Val-L-Met-L-His-L-Pro-L-Leu + H2O
-
 Human T-cell leukemia virus type I L-Lys-(7-methoxycoumarin-4-yl)acetyl-L-Ala-L-Pro-L-Gln-L-Val-L-Leu + 4-nitro-L-phenylalanine-L-Val-L-Met-L-His-L-Pro-L-Leu
-
 ?

Subunits

Subunits Comment Organism
? x * 13000, SDS-PAGE of recombinant enzyme Human T-cell leukemia virus type I

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.64
-
 N6-(7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Ala-L-Pro-L-Gln-L-Val-L-Leu-4-nitro-L-phenylalanine-L-Val-L-Met-L-His-L-Pro-L-Leu His-tagged recombinant protein, pH 5.3, 37°C Human T-cell leukemia virus type I
42.9
-
 N6-(7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Ala-L-Pro-L-Gln-L-Val-L-Leu-4-nitro-L-phenylalanine-L-Val-L-Met-L-His-L-Pro-L-Leu mutant L40I, pH 5.3, 37°C Human T-cell leukemia virus type I

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11.7
-
 N6-(7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Ala-L-Pro-L-Gln-L-Val-L-Leu-4-nitro-L-phenylalanine-L-Val-L-Met-L-His-L-Pro-L-Leu His-tagged recombinant protein, pH 5.3, 37°C Human T-cell leukemia virus type I
5860
-
 N6-(7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Ala-L-Pro-L-Gln-L-Val-L-Leu-4-nitro-L-phenylalanine-L-Val-L-Met-L-His-L-Pro-L-Leu mutant L40I, pH 5.3, 37°C Human T-cell leukemia virus type I