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Literature summary for 3.4.23.B8 extracted from

  • Herger, B.E.; Mariani, V.L.; Dennison, K.; Shuker, S.B.
    The 10 C-terminal residues of HTLV-I protease are not necessary for enzymatic activity (2004), Biochem. Biophys. Res. Commun., 320, 1306-1308.
    View publication on PubMed
Search for more literature for 3.4.23.B8

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzyme in Escherichia coli strain BL21(DE3) Human T-cell leukemia virus type I

Protein Variants

Protein Variants Comment Organism
L40I site-directed mutagenesis, modified wild-type protease Human T-cell leukemia virus type I
additional information construction of a L40I mutant enzyme lacking the 10 C-terminal amino acid residues, the mutant shows slightly increased activity compared to the wild-type enzyme Human T-cell leukemia virus type I

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0068
-
 dabsyl-(GABA)-PQVLPVMH-EDANS pH 5.2, 37°C, modified L40I mutant enzyme lacking the 10 C-terminal amino acid residues Human T-cell leukemia virus type I
0.01
-
 dabsyl-(GABA)-PQVLPVMH-EDANS pH 5.2, 37°C, modified L40I wild-type enzyme Human T-cell leukemia virus type I

Organism

Organism UniProt Comment Textmining
Human T-cell leukemia virus type I
-
 HTLV-I
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-(GABA)-PQVLPVMH-EDANS + H2O fluorogenic peptide substrate containing a MA/CA cleavage site Human T-cell leukemia virus type I dabsyl-(GABA)-PQVL + PVMH-EDANS
-
 ?
additional information the 10 C-terminal residues of HTLV-I protease are not necessary for enzymatic activity Human T-cell leukemia virus type I ?
-
 ?

Subunits

Subunits Comment Organism
More the 10 C-terminal residues of HTLV-I protease are not necessary for enzymatic activity, structure model of wild-type enzyme and mutant enzyme lacking the 10 C-terminal amino acid residues Human T-cell leukemia virus type I

Synonyms

Synonyms Comment Organism
HTLV-I protease
-
 Human T-cell leukemia virus type I
human T-lymphotropic virus type I protease
-
 Human T-cell leukemia virus type I
More belongs to the leukemic proteases Human T-cell leukemia virus type I

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Human T-cell leukemia virus type I

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0046
-
 dabsyl-(GABA)-PQVLPVMH-EDANS pH 5.2, 37°C, modified L40I mutant enzyme lacking the 10 C-terminal amino acid residues Human T-cell leukemia virus type I
0.0057
-
 dabsyl-(GABA)-PQVLPVMH-EDANS pH 5.2, 37°C, modified L40I wild-type enzyme Human T-cell leukemia virus type I

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
 assay at Human T-cell leukemia virus type I