EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   3.4.21.45 | -999 |
- |
- |
95489 |
| 3.4.21.45 | -999 |
- |
assay of complement component C3b and Cb4 cofactor function and endpoint assay described, complement component C3b and Cb4 cofactor function of M120I, G243D and R388H correspond to those of unmodified proteins, 30% activity retained in R299W mutants, no activity in the mutants D501N, D506V and I322T observed |
683892 |
| 3.4.21.45 | -999 |
- |
complement component C3 level measured in plasma, glomerular complement component C3 staining and complement profile in mice with deficiency of complement factor I indicated |
683705 |
| 3.4.21.45 | -999 |
- |
hemolytic complement activity test performed, plasma levels and capability to degrade complement components C4b and C3b analyzed in wild-type and mutant protein, kinetics shown, no major differences between mutant and wild-type protein identified |
683883 |
| 3.4.21.45 | -999 |
- |
keratinocytes stimulated with IFN-gamma (10-1000 U/ml), IL-1alpha (25-200 U/ml), IL-6 (250-1000 U/ml), TNF-alpha (100-1000 U/ml) or TGF-beta1, presence of complement factor I analyzed by monitoring cleavage of complement component C3b, assay with radiolabelled purified complement component C3b |
683884 |
| 3.4.21.45 | -999 |
- |
presence of complement factor I analyzed by RT-PCR and by monitoring cleavage of complemement components C3b and C4b, assay described, protein concentrations determined by ELISA |
683888 |
| 3.4.21.45 | -999 |
- |
presence of complement factor I analyzed by RT-PCR and by monitoring cleavage of complement components C3b and C4b, assay described, protein concentrations determined by ELISA, complement-dependent lysis of non-small cell lung cancer cells analyzed |
683888 |
| 3.4.21.45 | -999 |
- |
presence of complement factor I analyzed by RT-PCR and by monitoring cleavage of the complement components C3b and C4b, assay described, protein concentrations determined by ELISA, complement-dependent lysis of non-small cell lung cancer cells analyzed |
683888 |
| 3.4.21.45 | -999 |
- |
proteolytic activities of the native and partially deglycosylated forms analyzed, similar activities against complement component C3 indicated, charged glycans of complement factor I do not influence cofactor-substrate binding properties |
683180 |
