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Literature summary for 3.4.21.45 extracted from

  • Timar, K.K.; Junnikkala, S.; Dallos, A.; Jarva, H.; Bhuiyan, Z.A.; Meri, S.; Bos, J.D.; Asghar, S.S.
    Human keratinocytes produce the complement inhibitor factor I: Synthesis is regulated by interferon-gamma (2007), Mol. Immunol., 44, 2943-2949.
    View publication on PubMed
Search for more literature for 3.4.21.45

Application

Application Comment Organism
medicine studies on mutations of complement factor I Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, mutant and unmodified proteins Homo sapiens

Protein Variants

Protein Variants Comment Organism
G243D cofactor function of complement components C3b and Cb4 not affected Homo sapiens
M120I clinical data of aHUS-patients with complemment factor I mutation summarized, cofactor function of complement components C3b and Cb4 not affected Homo sapiens
R388H cofactor function of complement components C3b and Cb4 not affected Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 heavy chain, indicative of processing Homo sapiens
88000
-
 non-reduced form, SDS-PAGE Homo sapiens
88000
-
 reduced form, RRKR-linker peptide not cleaved, corresponds to pro-complemement factor I Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
complement component C3b + H2O Homo sapiens
-
 complement component C3c + ?
-
 ?
complement component C4b + H2O Homo sapiens
-
 complement component C4c + C4d
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P05156 encoded by CFI gene
-

Purification (Commentary)

Purification (Comment) Organism
recombinant and native proteins, gel filtration, SDS-PAGE Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
keratinocyte incubation of foreskin with thermolysin followed by trypsinization Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 keratinocytes stimulated with IFN-gamma (10-1000 U/ml), IL-1alpha (25-200 U/ml), IL-6 (250-1000 U/ml), TNF-alpha (100-1000 U/ml) or TGF-beta1, presence of complement factor I analyzed by monitoring cleavage of complement component C3b, assay with radiolabelled purified complement component C3b Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C3b + H2O
-
 Homo sapiens complement component C3c + ?
-
 ?
complement component C3b + H2O complement factor I production of human keratinocytes analyzed, with and without stimulation by IFN-gamma, IL-1alpha, IL-6, TNF-alpha, TGF-beta1 Homo sapiens complement component C3c + ?
-
 ?
complement component C4b + H2O
-
 Homo sapiens complement component C4c + C4d
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 C3b cleavage assay at Homo sapiens