EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   4.1.3.43 | 33.3 |
- |
4-hydroxy-2-oxohexanoate |
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C |
721659 |
| 4.1.3.43 | 46.1 |
- |
4-hydroxy-2-oxopentanoate |
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C |
721659 |
| 4.1.3.43 | 7.8 |
- |
4-hydroxy-2-oxopentanoate |
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C |
721659 |
| 4.1.3.43 | 10.6 |
- |
4-hydroxy-2-oxohexanoate |
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C |
721659 |
| 4.1.3.43 | 0.45 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
| 4.1.3.43 | 0.34 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
| 4.1.3.43 | -999 |
- |
more |
highest catalytic efficiency of BphI occurs when NADH is present, which is 25fold higher than the value obtained without nucleotides |
721615 |
| 4.1.3.43 | 0.00047 |
- |
pentaldehyde |
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C |
702377 |
| 4.1.3.43 | 0.0005 |
- |
Isobutyraldehyde |
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C |
702377 |
| 4.1.3.43 | 0.0022 |
- |
Butyraldehyde |
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C |
702377 |
