BRENDA - Enzyme Database
show all sequences of 4.1.3.43

Probing the molecular basis of substrate specificity, stereospecificity, and catalysis in the class II pyruvate aldolase, BphI

Baker, P.; Carere, J.; Seah, S.Y.; Biochemistry 50, 3559-3569 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
bphI previous to mutagenesis cloned into plasmid pBTL4-T7, Escherichia coli
Paraburkholderia xenovorans
Engineering
Protein Variants
Commentary
Organism
H20A
mutant generated by site-directed mutagenesis, pH profiles show the dependence of enzyme activity on hydroxide concentration
Paraburkholderia xenovorans
H20S
mutant generated by site-directed mutagenesis, pH profiles show the dependence of enzyme activity on hydroxide concentration
Paraburkholderia xenovorans
L87A
mutant generated by site-directed mutagenesis, variant exhibits a 40fold preference for propionaldehyde over acetaldehyde in contrast to wild type with similar specificities for acetaldehyde and propionaldehyde
Paraburkholderia xenovorans
L89A
mutant generated by site-directed mutagenesis, specificity constant of this variant in the aldol addition reaction using pentaldehyde is increased approx. 50fold, making it more catalytically efficient for pentaldehyde utilization compared to the wild-type utilization of the natural substrate, acetaldehyde
Paraburkholderia xenovorans
R16A
mutant generated by site-directed mutagenesis, no detectable aldol cleavage and pyruvate R-proton exchange, supporting the role of Arg-16 in stabilizing a pyruvate enolate intermediate
Paraburkholderia xenovorans
Y290F
mutant generated by site-directed mutagenesis, mutation results in a loss of stereochemical control as the variant is able to utilize substrates with R and S configurations at C4 with similar kinetic parameters
Paraburkholderia xenovorans
Y290S
mutant generated by site-directed mutagenesis, mutation results in a loss of stereochemical control as the variant is able to utilize substrates with R and S configurations at C4 with similar kinetic parameters
Paraburkholderia xenovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
value not detectable for R16A variant of BphI
Paraburkholderia xenovorans
0.012
-
(4R)-4-hydroxy-2-oxopentanoate
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.013
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.013
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C; Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.089
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.222
-
(4S)-4-hydroxy-2-oxopentanoate
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.228
-
(4S)-4-hydroxy-2-oxopentanoate
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
11
-
pyruvate
L89A variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
13
-
pyruvate
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
16.8
-
propionaldehyde
L87A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
20.2
-
pyruvate
L87A variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
24.8
-
pentaldehyde
L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
33.3
-
pentaldehyde
L87A/L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
40.2
-
propionaldehyde
L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
49.2
-
Butyraldehyde
L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
64.2
-
acetaldehyde
wild-type BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
80.5
-
acetaldehyde
L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
136
-
propionaldehyde
wild-type BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
289
-
Butyraldehyde
L87A/L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
323
-
Butyraldehyde
L87A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
Organism
Organism
UniProt
Commentary
Textmining
Paraburkholderia xenovorans
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(4R)-4-hydroxy-2-oxopentanoate
-
714244
Paraburkholderia xenovorans
pyruvate + acetaldehyde
-
-
-
?
(4S)-4-hydroxy-2-oxopentanoate
-
714244
Paraburkholderia xenovorans
pyruvate + acetaldehyde
-
-
-
?
pyruvate + acetaldehyde
-
714244
Paraburkholderia xenovorans
(4R)-4-hydroxy-2-oxopentanoate
-
-
-
?
pyruvate + acetaldehyde
-
714244
Paraburkholderia xenovorans
(4S)-4-hydroxy-2-oxopentanoate
-
-
-
?
pyruvate + butyraldehyde
-
714244
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + hexaldehyde
-
714244
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + pentaldehyde
-
714244
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + propionaldehyde
reaction catalyzed by BphI variants Y290F and Y290S
714244
Paraburkholderia xenovorans
(4R)-4-hydroxy-2-oxohexanoate
-
-
-
?
pyruvate + propionaldehyde
-
714244
Paraburkholderia xenovorans
(4S)-4-hydroxy-2-oxohexanoate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
BphI
-
Paraburkholderia xenovorans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
value not detectable for R16A variant of BphI
Paraburkholderia xenovorans
0.089
-
(4S)-4-hydroxy-2-oxopentanoate
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.099
-
(4S)-4-hydroxy-2-oxopentanoate
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.13
-
pentaldehyde
L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.131
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.132
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.17
-
(4R)-4-hydroxy-2-oxopentanoate
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.171
-
(4S)-4-hydroxy-2-oxopentanoate
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.26
-
Butyraldehyde
L87A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.29
-
propionaldehyde
L87A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.3
-
Butyraldehyde
L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.32
-
pyruvate
L87A variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.58
-
pentaldehyde
L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.63
-
propionaldehyde
L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.64
-
Butyraldehyde
L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.64
-
pyruvate
L89A variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.86
-
acetaldehyde
wild-type BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.94
-
acetaldehyde
L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
1.2
-
pyruvate
wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
1.79
-
propionaldehyde
wild-type BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
4.07
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
bphI previous to mutagenesis cloned into plasmid pBTL4-T7, Escherichia coli
Paraburkholderia xenovorans
Engineering (protein specific)
Protein Variants
Commentary
Organism
H20A
mutant generated by site-directed mutagenesis, pH profiles show the dependence of enzyme activity on hydroxide concentration
Paraburkholderia xenovorans
H20S
mutant generated by site-directed mutagenesis, pH profiles show the dependence of enzyme activity on hydroxide concentration
Paraburkholderia xenovorans
L87A
mutant generated by site-directed mutagenesis, variant exhibits a 40fold preference for propionaldehyde over acetaldehyde in contrast to wild type with similar specificities for acetaldehyde and propionaldehyde
Paraburkholderia xenovorans
L89A
mutant generated by site-directed mutagenesis, specificity constant of this variant in the aldol addition reaction using pentaldehyde is increased approx. 50fold, making it more catalytically efficient for pentaldehyde utilization compared to the wild-type utilization of the natural substrate, acetaldehyde
Paraburkholderia xenovorans
R16A
mutant generated by site-directed mutagenesis, no detectable aldol cleavage and pyruvate R-proton exchange, supporting the role of Arg-16 in stabilizing a pyruvate enolate intermediate
Paraburkholderia xenovorans
Y290F
mutant generated by site-directed mutagenesis, mutation results in a loss of stereochemical control as the variant is able to utilize substrates with R and S configurations at C4 with similar kinetic parameters
Paraburkholderia xenovorans
Y290S
mutant generated by site-directed mutagenesis, mutation results in a loss of stereochemical control as the variant is able to utilize substrates with R and S configurations at C4 with similar kinetic parameters
Paraburkholderia xenovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
value not detectable for R16A variant of BphI
Paraburkholderia xenovorans
0.012
-
(4R)-4-hydroxy-2-oxopentanoate
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.013
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.013
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C; Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.089
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.222
-
(4S)-4-hydroxy-2-oxopentanoate
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.228
-
(4S)-4-hydroxy-2-oxopentanoate
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
11
-
pyruvate
L89A variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
13
-
pyruvate
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
16.8
-
propionaldehyde
L87A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
20.2
-
pyruvate
L87A variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
24.8
-
pentaldehyde
L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
33.3
-
pentaldehyde
L87A/L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
40.2
-
propionaldehyde
L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
49.2
-
Butyraldehyde
L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
64.2
-
acetaldehyde
wild-type BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
80.5
-
acetaldehyde
L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
136
-
propionaldehyde
wild-type BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
289
-
Butyraldehyde
L87A/L89A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
323
-
Butyraldehyde
L87A variant of BphI, app. Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(4R)-4-hydroxy-2-oxopentanoate
-
714244
Paraburkholderia xenovorans
pyruvate + acetaldehyde
-
-
-
?
(4S)-4-hydroxy-2-oxopentanoate
-
714244
Paraburkholderia xenovorans
pyruvate + acetaldehyde
-
-
-
?
pyruvate + acetaldehyde
-
714244
Paraburkholderia xenovorans
(4R)-4-hydroxy-2-oxopentanoate
-
-
-
?
pyruvate + acetaldehyde
-
714244
Paraburkholderia xenovorans
(4S)-4-hydroxy-2-oxopentanoate
-
-
-
?
pyruvate + butyraldehyde
-
714244
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + hexaldehyde
-
714244
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + pentaldehyde
-
714244
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + propionaldehyde
reaction catalyzed by BphI variants Y290F and Y290S
714244
Paraburkholderia xenovorans
(4R)-4-hydroxy-2-oxohexanoate
-
-
-
?
pyruvate + propionaldehyde
-
714244
Paraburkholderia xenovorans
(4S)-4-hydroxy-2-oxohexanoate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
value not detectable for R16A variant of BphI
Paraburkholderia xenovorans
0.089
-
(4S)-4-hydroxy-2-oxopentanoate
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.099
-
(4S)-4-hydroxy-2-oxopentanoate
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.13
-
pentaldehyde
L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.131
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.132
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.17
-
(4R)-4-hydroxy-2-oxopentanoate
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.171
-
(4S)-4-hydroxy-2-oxopentanoate
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.26
-
Butyraldehyde
L87A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.29
-
propionaldehyde
L87A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.3
-
Butyraldehyde
L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.32
-
pyruvate
L87A variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.58
-
pentaldehyde
L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.63
-
propionaldehyde
L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.64
-
Butyraldehyde
L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.64
-
pyruvate
L89A variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.86
-
acetaldehyde
wild-type BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.94
-
acetaldehyde
L89A variant of BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
1.2
-
pyruvate
wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
1.79
-
propionaldehyde
wild-type BphI, app. kcat-value, pH 8.0, 25C
Paraburkholderia xenovorans
4.07
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
General Information
General Information
Commentary
Organism
metabolism
enzyme found in the polychlorinated biphenyls (PCBs) degradation pathway
Paraburkholderia xenovorans
General Information (protein specific)
General Information
Commentary
Organism
metabolism
enzyme found in the polychlorinated biphenyls (PCBs) degradation pathway
Paraburkholderia xenovorans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
value not detectable for R16A variant of BphI
Paraburkholderia xenovorans
0.00001
-
hexaldehyde
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25C; wild-type BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0001
-
hexaldehyde
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00013
-
hexaldehyde
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00033
-
pentaldehyde
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00042
-
acetaldehyde
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00047
-
pentaldehyde
wild-type BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00049
-
acetaldehyde
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00079
-
Butyraldehyde
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00104
-
Butyraldehyde
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0022
-
Butyraldehyde
wild-type BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0038
-
pentaldehyde
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00673
-
propionaldehyde
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0117
-
acetaldehyde
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.012
-
(4S)-4-hydroxy-2-oxopentanoate
R16K variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.0129
-
Butyraldehyde
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0132
-
propionaldehyde
wild-type BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0134
-
acetaldehyde
wild-type BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0156
-
propionaldehyde
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0159
-
pyruvate
L87A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.0175
-
propionaldehyde
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0233
-
pentaldehyde
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0587
-
pyruvate
L89A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.0915
-
pyruvate
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.34
-
(4S)-4-hydroxy-2-oxopentanoate
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.45
-
(4S)-4-hydroxy-2-oxopentanoate
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
10.4
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
12.6
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
13.4
-
(4S)-4-hydroxy-2-oxopentanoate
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
14.1
-
(4R)-4-hydroxy-2-oxopentanoate
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
44.7
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
value not detectable for R16A variant of BphI
Paraburkholderia xenovorans
0.00001
-
hexaldehyde
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25C; wild-type BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0001
-
hexaldehyde
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00013
-
hexaldehyde
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00033
-
pentaldehyde
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00042
-
acetaldehyde
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00047
-
pentaldehyde
wild-type BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00049
-
acetaldehyde
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00079
-
Butyraldehyde
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00104
-
Butyraldehyde
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0022
-
Butyraldehyde
wild-type BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0038
-
pentaldehyde
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.00673
-
propionaldehyde
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0117
-
acetaldehyde
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.012
-
(4S)-4-hydroxy-2-oxopentanoate
R16K variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.0129
-
Butyraldehyde
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0132
-
propionaldehyde
wild-type BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0134
-
acetaldehyde
wild-type BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0156
-
propionaldehyde
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0159
-
pyruvate
L87A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.0175
-
propionaldehyde
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0233
-
pentaldehyde
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25C
Paraburkholderia xenovorans
0.0587
-
pyruvate
L89A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.0915
-
pyruvate
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.34
-
(4S)-4-hydroxy-2-oxopentanoate
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
0.45
-
(4S)-4-hydroxy-2-oxopentanoate
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
10.4
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
12.6
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
13.4
-
(4S)-4-hydroxy-2-oxopentanoate
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
14.1
-
(4R)-4-hydroxy-2-oxopentanoate
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
44.7
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25C
Paraburkholderia xenovorans
Other publictions for EC 4.1.3.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721659
Baker
Protein-protein interactions a ...
Thermus thermophilus
Biochemistry
51
1942-1952
2012
-
-
1
-
1
-
-
16
-
-
7
-
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
1
15
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
16
-
-
7
-
-
-
-
1
-
-
-
-
4
1
-
-
1
15
-
-
-
-
-
1
1
-
15
15
722458
Baker
Rational design of stereoselec ...
Paraburkholderia xenovorans
J. Am. Chem. Soc.
134
507-513
2012
-
-
1
-
6
-
-
24
-
-
-
-
-
1
-
-
-
-
-
-
-
-
10
-
1
-
-
-
24
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
24
-
-
-
-
-
-
-
-
31
31
714244
Baker
Probing the molecular basis of ...
Paraburkholderia xenovorans
Biochemistry
50
3559-3569
2011
-
-
1
-
7
-
-
20
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
1
-
-
-
21
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
21
-
-
-
-
-
1
1
-
31
31
721651
Carere
Investigating the molecular de ...
Paraburkholderia xenovorans
Biochemistry
50
8407-8416
2011
-
-
1
-
10
-
-
19
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
19
-
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
19
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
19
-
-
-
-
-
1
1
-
-
-
702377
Wang
Comparison of two metal-depend ...
Paraburkholderia xenovorans
Biochemistry
49
3774-3782
2010
-
-
-
-
-
-
5
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
6
-
1
-
-
-
4
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
5
6
5
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
-
-
-
6
6
721615
Baker
Characterization of an aldolas ...
Paraburkholderia xenovorans
Biochemistry
48
6551-6558
2009
1
-
1
-
-
-
6
5
-
-
3
1
-
1
-
-
1
-
-
-
-
1
5
1
1
-
-
-
5
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
6
1
5
-
-
3
1
-
-
-
1
-
-
-
1
5
1
-
-
-
5
-
-
-
-
-
1
1
-
5
5