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show all sequences of 4.1.3.43

Comparison of two metal-dependent pyruvate aldolases related by convergent evolution: substrate specificity, kinetic mechanism, and substrate channeling

Wang, W.; Baker, P.; Seah, S.Y.; Biochemistry 49, 3774-3782 (2010)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-oxobutanoate
competitive inhibition
Paraburkholderia xenovorans
2-Oxopentanoate
competitive inhibition
Paraburkholderia xenovorans
4-methyl-2-oxopentanoate
competitive inhibition
Paraburkholderia xenovorans
glyoxylate
competitive inhibition
Paraburkholderia xenovorans
pyruvate
competitive inhibition
Paraburkholderia xenovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
14.8
-
pyruvate
steady-state kinetic parameter, pH 8.0 and 25°C
Paraburkholderia xenovorans
64.3
-
acetaldehyde
app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
67
-
acetaldehyde
steady-state kinetic parameter, pH 8.0 and 25°C
Paraburkholderia xenovorans
124.6
-
glycolaldehyde
app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
135.9
-
propionaldehyde
app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
cofactor
Paraburkholderia xenovorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paraburkholderia xenovorans
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyruvate + acetaldehyde
-
702377
Paraburkholderia xenovorans
(S)-4-hydroxy-2-oxopentanoate
-
-
-
?
pyruvate + butyraldehyde
-
702377
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + glycolaldehyde
-
702377
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + isobutyraldehyde
-
702377
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + pentaldehyde
-
702377
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + propionaldehyde
-
702377
Paraburkholderia xenovorans
?
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.4
-
glycolaldehyde
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.86
-
acetaldehyde
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
1.04
-
pyruvate
steady-state kinetic parameter, with acetaldehyde as aldehyde donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
1.79
-
propionaldehyde
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.21
-
glyoxylate
pH 8.0, 25°C
Paraburkholderia xenovorans
0.34
-
2-oxobutanoate
pH 8.0, 25°C
Paraburkholderia xenovorans
0.55
-
pyruvate
pH 8.0, 25°C
Paraburkholderia xenovorans
1.09
-
2-Oxopentanoate
pH 8.0, 25°C
Paraburkholderia xenovorans
2.71
-
4-methyl-2-oxopentanoate
pH 8.0, 25°C
Paraburkholderia xenovorans
10.3
-
pyruvate
pH 8.0, 25°C
Paraburkholderia xenovorans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-oxobutanoate
competitive inhibition
Paraburkholderia xenovorans
2-Oxopentanoate
competitive inhibition
Paraburkholderia xenovorans
4-methyl-2-oxopentanoate
competitive inhibition
Paraburkholderia xenovorans
glyoxylate
competitive inhibition
Paraburkholderia xenovorans
pyruvate
competitive inhibition
Paraburkholderia xenovorans
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.21
-
glyoxylate
pH 8.0, 25°C
Paraburkholderia xenovorans
0.34
-
2-oxobutanoate
pH 8.0, 25°C
Paraburkholderia xenovorans
0.55
-
pyruvate
pH 8.0, 25°C
Paraburkholderia xenovorans
1.09
-
2-Oxopentanoate
pH 8.0, 25°C
Paraburkholderia xenovorans
2.71
-
4-methyl-2-oxopentanoate
pH 8.0, 25°C
Paraburkholderia xenovorans
10.3
-
pyruvate
pH 8.0, 25°C
Paraburkholderia xenovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
14.8
-
pyruvate
steady-state kinetic parameter, pH 8.0 and 25°C
Paraburkholderia xenovorans
64.3
-
acetaldehyde
app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
67
-
acetaldehyde
steady-state kinetic parameter, pH 8.0 and 25°C
Paraburkholderia xenovorans
124.6
-
glycolaldehyde
app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
135.9
-
propionaldehyde
app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
cofactor
Paraburkholderia xenovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyruvate + acetaldehyde
-
702377
Paraburkholderia xenovorans
(S)-4-hydroxy-2-oxopentanoate
-
-
-
?
pyruvate + butyraldehyde
-
702377
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + glycolaldehyde
-
702377
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + isobutyraldehyde
-
702377
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + pentaldehyde
-
702377
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + propionaldehyde
-
702377
Paraburkholderia xenovorans
?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.4
-
glycolaldehyde
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.86
-
acetaldehyde
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
1.04
-
pyruvate
steady-state kinetic parameter, with acetaldehyde as aldehyde donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
1.79
-
propionaldehyde
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.00047
-
pentaldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.0005
-
Isobutyraldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.0022
-
Butyraldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.0037
-
glycolaldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.0132
-
propionaldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.0134
-
acetaldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.00047
-
pentaldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.0005
-
Isobutyraldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.0022
-
Butyraldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.0037
-
glycolaldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.0132
-
propionaldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
0.0134
-
acetaldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Paraburkholderia xenovorans
Other publictions for EC 4.1.3.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721659
Baker
Protein-protein interactions a ...
Thermus thermophilus
Biochemistry
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1942-1952
2012
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-
1
-
1
-
-
16
-
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7
-
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4
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1
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4
1
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1
15
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1
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1
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16
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7
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1
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4
1
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1
15
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1
1
-
15
15
722458
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Paraburkholderia xenovorans
J. Am. Chem. Soc.
134
507-513
2012
-
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1
-
6
-
-
24
-
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1
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10
-
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-
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24
-
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1
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6
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24
-
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10
-
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-
-
24
-
-
-
-
-
-
-
-
31
31
714244
Baker
Probing the molecular basis of ...
Paraburkholderia xenovorans
Biochemistry
50
3559-3569
2011
-
-
1
-
7
-
-
20
-
-
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1
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9
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21
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1
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7
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20
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-
9
-
-
-
-
21
-
-
-
-
-
1
1
-
31
31
721651
Carere
Investigating the molecular de ...
Paraburkholderia xenovorans
Biochemistry
50
8407-8416
2011
-
-
1
-
10
-
-
19
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
19
-
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
19
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
19
-
-
-
-
-
1
1
-
-
-
702377
Wang
Comparison of two metal-depend ...
Paraburkholderia xenovorans
Biochemistry
49
3774-3782
2010
-
-
-
-
-
-
5
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
5
6
5
-
1
-
-
-
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-
-
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-
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6
-
-
-
-
4
-
-
-
-
-
-
-
-
6
6
721615
Baker
Characterization of an aldolas ...
Paraburkholderia xenovorans
Biochemistry
48
6551-6558
2009
1
-
1
-
-
-
6
5
-
-
3
1
-
1
-
-
1
-
-
-
-
1
5
1
-
-
-
5
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
6
1
5
-
-
3
1
-
-
-
1
-
-
-
1
5
1
-
-
-
5
-
-
-
-
-
1
1
-
5
5