EC Number   |
Reference |
|---|
   4.1.99.13 | protein is isolated from whole cell extracts from Xenopus laevis, purified by using a sepharose column and a UV-damaged DNA affinity column |
682264 |
| 4.1.99.13 | purified to near homogeneity from Drosophila embryonic cells by using NH4SO4 precipitation, SP sepharose column, UV DNA affinity beads, heparin sepharose column and Mono S column |
682049 |
| 4.1.99.13 | recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, and recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain C41(DE3) by nickel affinity and heparin affinity chromatography |
747287 |
| 4.1.99.13 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration under dim light conditions or in darkness |
728696 |
| 4.1.99.13 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity and heparin affinity chromatography, followed by gel filtration |
747746 |
| 4.1.99.13 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain ER2566 by nickel affinity chromatography and gel filtration |
747528 |
| 4.1.99.13 | recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity and heparin affinity chromatography |
726991 |
| 4.1.99.13 | recombinant wild-type and mutant enzymes from Escherichia coli |
749067 |
