Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | for activation of Xenopus (6-4) PHR, FADox is first converted to a neutral radical form by light-induced one-electron and one-proton transfers and then into a fully reduced form by light-induced one electron transfer, four different redox states for the FAD chromophore of PHR, mechanism, overview | Xenopus laevis |
Cloned (Comment) | Organism |
---|---|
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6-4) photoproduct (in DNA) | Xenopus laevis | - |
2 pyrimidine residues (in DNA) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity and heparin affinity chromatography | Xenopus laevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6-4) photoproduct (in DNA) | - |
Xenopus laevis | 2 pyrimidine residues (in DNA) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(6-4) photolyase | - |
Xenopus laevis |
(6-4) PHR | - |
Xenopus laevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | for activation of Xenopus (6-4) PHR, FADox is first converted to a neutral radical form by light-induced one-electron and one-proton transfers and then into a fully reduced form by light-induced one electron transfer, mechanism, overview | Xenopus laevis | |
additional information | the enzyme protein expressed in Escherichia coli does not bind a second chromophore, such as 5,10-methenyltetrahydrofolate, MTHF | Xenopus laevis |