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Literature summary for 4.1.99.13 extracted from

  • Yamada, D.; Zhang, Y.; Iwata, T.; Hitomi, K.; Getzoff, E.D.; Kandori, H.
    Fourier-transform infrared study of the photoactivation process of Xenopus (6-4) photolyase (2012), Biochemistry, 51, 5774-5783.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information for activation of Xenopus (6-4) PHR, FADox is first converted to a neutral radical form by light-induced one-electron and one-proton transfers and then into a fully reduced form by light-induced one electron transfer, four different redox states for the FAD chromophore of PHR, mechanism, overview Xenopus laevis

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6-4) photoproduct (in DNA) Xenopus laevis
-
2 pyrimidine residues (in DNA)
-
?

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity and heparin affinity chromatography Xenopus laevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6-4) photoproduct (in DNA)
-
Xenopus laevis 2 pyrimidine residues (in DNA)
-
?

Synonyms

Synonyms Comment Organism
(6-4) photolyase
-
Xenopus laevis
(6-4) PHR
-
Xenopus laevis

Cofactor

Cofactor Comment Organism Structure
FAD for activation of Xenopus (6-4) PHR, FADox is first converted to a neutral radical form by light-induced one-electron and one-proton transfers and then into a fully reduced form by light-induced one electron transfer, mechanism, overview Xenopus laevis
additional information the enzyme protein expressed in Escherichia coli does not bind a second chromophore, such as 5,10-methenyltetrahydrofolate, MTHF Xenopus laevis