4.1.99.13	-	693832, 703212, 716329	
4.1.99.13	(6-4) photoproduct DNA photolyase activity is detected in Crotalus atrox fibroblast. Activity is considerably enhanced when a UV-damaged DNA affinity column is used for purification. However, the activity is unstable and it is lost during purification or upon storage at -20° or -70°C for 2-3 months	682264	
4.1.99.13	by fractionation of crude cell extracts with Heparin agarose and UV DNA affinity column chromatography	682086	
4.1.99.13	by using a glutathione sepharose column and a Hi Trap Q column. Concentrated fusion protein is cleaved with thrombin from bovine plasma by incubation overnight at 4°C. For chromophore determination, the eluate from the glutathione sepharose column is purified through a Q sepharose column, omitting gel filtration procedure, and concentrated by ultrafiltration	682048	
4.1.99.13	by using a glutathione-sepharose column and UV-irradiated DNA affinity column, fusion protein is cleaved with thrombin	682154	
4.1.99.13	by using affinity chromatography and UV-irradiated DNA attached beads	682855	
4.1.99.13	by using glutathione-sepharose columns	679920, 680605	
4.1.99.13	fusion protein is applied to amylose column. At this point the protein is above 90% pure. Further purification can be obtained by applying the eluted material to a 10 ml heparin-agarose column. Maltose-binding protein is removed by treatment with factor Xa protease	681765	
4.1.99.13	fusion protein is purified through a 20-ml amylose column and through heparin-agarose column	680604	
4.1.99.13	NF-1 is isolated from nuclear extracts prepared from HeLa cell culture using a heparin column, an anion exchange Fractogel EMD TMAE-650 (S) column and a FPLC MonoS HR5/5 column	682153	
4.1.99.13	protein is isolated from whole cell extracts from Xenopus laevis, purified by using a sepharose column and a UV-damaged DNA affinity column	682264	
4.1.99.13	purified to near homogeneity from Drosophila embryonic cells by using NH4SO4 precipitation, SP sepharose column, UV DNA affinity beads, heparin sepharose column and Mono S column	682049	
4.1.99.13	recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, and recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain C41(DE3) by nickel affinity and heparin affinity chromatography	747287	
4.1.99.13	recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration under dim light conditions or in darkness	728696	
4.1.99.13	recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity and heparin affinity chromatography, followed by gel filtration	747746	
4.1.99.13	recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain ER2566 by nickel affinity chromatography and gel filtration	747528	
4.1.99.13	recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity and heparin affinity chromatography	726991	
4.1.99.13	recombinant wild-type and mutant enzymes from Escherichia coli	749067