   3.5.1.100 | synthesis |
expression of amidase in Escherichia coli using a T7 promoter. Amidase activity of the engineered Escherichia coli strain reaches 2963 U/l in a 5-l bioreactor, and can be further increased to 5255 U/l in a 100-l bioreactor. Using cell-free extract prepared from 1 kg (wet cell weight) of recombinant cells as catalyst, 60 kg of R,S-DMCPCA is resolved into S-DMCPCA (28.6 kg) and R-2,2-dimethylcyclopropanecarboxylic acid (31.7 kg) in 18 h, and the enantiomeric excess (ee) value of S-DMCPCA reaches 99.32%. 20.5 kg of pure S-DMCPCA is obtained after concentration and crystallization, corresponding to a total yield of 34.2% from R,S-DMCPCA |
720957 |
