Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.100 extracted from

  • Yang, Z.; Ni, Y.; Lu, Z.; Liao, X.; Zheng, Y.; Sun, Z.
    Industrial production of S-2,2-dimethylcyclopropanecarboxamide with a novel recombinant R-amidase from Delftia tsuruhatensis (2011), Process Biochem., 46, 182-187.
No PubMed abstract available
Search for more literature for 3.5.1.100

Application

Application Comment Organism
synthesis expression of amidase in Escherichia coli using a T7 promoter. Amidase activity of the engineered Escherichia coli strain reaches 2963 U/l in a 5-l bioreactor, and can be further increased to 5255 U/l in a 100-l bioreactor. Using cell-free extract prepared from 1 kg (wet cell weight) of recombinant cells as catalyst, 60 kg of R,S-DMCPCA is resolved into S-DMCPCA (28.6 kg) and R-2,2-dimethylcyclopropanecarboxylic acid (31.7 kg) in 18 h, and the enantiomeric excess (ee) value of S-DMCPCA reaches 99.32%. 20.5 kg of pure S-DMCPCA is obtained after concentration and crystallization, corresponding to a total yield of 34.2% from R,S-DMCPCA Delftia tsuruhatensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Delftia tsuruhatensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49990
-
 x * 50000, SDS-PAGE, x * 49990, calculated Delftia tsuruhatensis
50000
-
 x * 50000, SDS-PAGE, x * 49990, calculated Delftia tsuruhatensis

Organism

Organism UniProt Comment Textmining
Delftia tsuruhatensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2,2-dimethylcyclopropanecarboxamide + H2O
-
 Delftia tsuruhatensis (R)-2,2-dimethylcyclopropanecarboxylic acid amidase exhibits strict R-selectivity towards 2,2-dimethylcyclopropanecarboxamide ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE, x * 49990, calculated Delftia tsuruhatensis

pI Value

Organism Comment pI Value Maximum pI Value
Delftia tsuruhatensis calculated
-
 6.7