EC Number |
Natural Substrates |
---|
3.2.1.207 | Glc2Man9GlcNAc2 (G2M9)-protein + H2O |
glucosidase II plays a key role in glycoprotein processing in the endoplasmic reticulum. This enzyme trims two alpha-1,3-linked glucose residues, Glcalpha1,3Glc (cleavage 1) and Glcalpha1,3Man (cleavage 2) from high-mannose type Glc2Man9GlcNAc2 (G2M9)-proteins. A crowded milieu that contains bovine serum albumin greatly enhances the second trimming step (cleavage 2), which deglucosylates Glc1Man9GlcNAc2, but not the first trimming step (cleavage 1), which removes the terminal glucose residue from Glc2Man9GlcNAc2 |
3.2.1.207 | Glc2Man9GlcNAc2 + H2O |
- |
3.2.1.207 | Glc2Man9GlcNAc2 + H2O |
involved in the Calnexin Cycle |
3.2.1.207 | Glc2Man9GlcNAc2 + H2O |
processing of asparagine-linked oligosaccharides |
3.2.1.207 | Glc2Man9GlcNAc2 + H2O |
removal of glucose residues allows newly synthesized glycoproteins to interact with calnexin and calreticulin, that are part of the chaperone mechanism |
3.2.1.207 | Glc2Man9GlcNAc2 + H2O |
i.e. G1M9 |
3.2.1.207 | GlcMan9GlcNAc2 + H2O |
- |
3.2.1.207 | GlcMan9GlcNAc2 + H2O |
G2M9 |
3.2.1.207 | more |
Catalyzes the hydrolysis of the inner two alpha-1,3-linked glucose residues present in all N-linked immature oligosaccharides, associates with and glycosylates the protein CD45, possible role in CD45 regulation |
3.2.1.207 | more |
involved in early glycoprotein biogenesis, catalyzes the hydrolysis of two alpha-1,3-linked glucose residues present on all Asn-linked precursor oligosaccharides |