EC Number   |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
|---|
   3.4.14.10 | -999 |
- |
amino acid sequence around the active site |
647140 |
| 3.4.14.10 | -999 |
- |
two TPPII species of 53000 Da and 142000 Da are detected by SDS-PAGE. The subunits assemble into higher order complexes of 5 to 9 MD |
666630 |
| 3.4.14.10 | 76000 |
- |
SDS-PAGE |
699739 |
| 3.4.14.10 | 80000 |
- |
mature enzyme, estimated from amino acid sequence |
699739 |
| 3.4.14.10 | 135000 |
- |
x * 135000, SDS-PAGE under reducing conditions |
647141, 647142, 647143 |
| 3.4.14.10 | 138000 |
- |
x * 138000 |
678441, 679644 |
| 3.4.14.10 | 138000 |
- |
x * 138000, the subunits form an active complex with a MW of 1000000 Da, the complex can sponateously dissociate in vitro into dimers which retain 1/10th of the original specific activity. The dissociated enzyme can reassociate at elevated temperatures, provided the protein concentration is sufficiently high. This reassociation is accompanied by a reactivation |
647152 |
| 3.4.14.10 | 145000 |
- |
His-tagged TPP II, SDS-PAGE |
696417 |
| 3.4.14.10 | 150000 |
- |
gel filtration |
647155 |
| 3.4.14.10 | 1000000 |
- |
value above, gel filtration |
647139, 647148 |
