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Literature summary for 3.4.14.10 extracted from

  • Lindas, A.C.; Eriksson, S.; Jozsa, E.; Tomkinson, B.
    Investigation of a role for Glu-331 and Glu-305 in substrate binding of tripeptidyl-peptidase II (2008), Biochim. Biophys. Acta, 1784, 1899-1907.
    View publication on PubMed
Search for more literature for 3.4.14.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Mus musculus

Protein Variants

Protein Variants Comment Organism
E305K the mutant shows extremely low activity Mus musculus
E305Q the mutant shows extremely low activity Mus musculus
E331K the catalytic efficiency is reduced 20000fold for the E331K variant compared to the wild type enzyme Mus musculus
E331Q the catalytic efficiency is reduced 400fold for the E331Q variant compared to the wild type enzyme Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
butabindide efficient, competitive inhibitor of TPP II Mus musculus
isovaleryl-L-Leu-L-Arg-L-Arg-L-Ala-L-Ser-L-Val-L-Ala
-
 Mus musculus
L-Val-L-Leu-L-Arg-L-Arg-L-Ala-L-Ser-L-Val-L-Ala
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 L-Ala-L-Ala-L-Phe-p-nitroanilide wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, containing 2 mM dithiothreitol, and 15% glycerol, at 37°C Mus musculus
1
-
 L-Ala-L-Ala-L-Phe-p-nitroanilide mutant enzyme E331K, Km above 1.0 mM, in 100 mM potassium phosphate buffer, pH 7.5, containing 2 mM dithiothreitol, and 15% glycerol, at 37°C Mus musculus
1
-
 L-Ala-L-Ala-L-Phe-p-nitroanilide mutant enzyme E331Q, Km above 1.0 mM, in 100 mM potassium phosphate buffer, pH 7.5, containing 2 mM dithiothreitol, and 15% glycerol, at 37°C Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
 His-tagged TPP II, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q64514
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose 6 column chromatography Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.068
-
 recombinant enzyme from cell lysate, at 37°C Mus musculus
12
-
 after 8fold purification, at 37°C Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-L-Ala-L-Phe-p-nitroanilide + H2O
-
 Mus musculus L-Ala-L-Ala-L-Phe + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
TPP II
-
 Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000011
-
 butabindide wild type enzyme, using 0.2 mM of substrate L-Ala-L-Ala-L-Phe-p-nitroanilide, in 100 mM potassium phosphate buffer, pH 7.5, containing 2 mM dithiothreitol, and 15% glycerol, at 37°C Mus musculus
0.000015
-
 butabindide wild type enzyme, using 0.02 mM of substrate L-Ala-L-Ala-L-Phe-p-nitroanilide, in 100 mM potassium phosphate buffer, pH 7.5, containing 2 mM dithiothreitol, and 15% glycerol, at 37°C Mus musculus
0.001
-
 L-Val-L-Leu-L-Arg-L-Arg-L-Ala-L-Ser-L-Val-L-Ala wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, containing 2 mM dithiothreitol, and 15% glycerol, at 37°C Mus musculus
0.14
-
 butabindide mutant enzyme E331Q, in 100 mM potassium phosphate buffer, pH 7.5, containing 2 mM dithiothreitol, and 15% glycerol, at 37°C Mus musculus
2
-
 L-Val-L-Leu-L-Arg-L-Arg-L-Ala-L-Ser-L-Val-L-Ala mutant enzyme E331Q, in 100 mM potassium phosphate buffer, pH 7.5, containing 2 mM dithiothreitol, and 15% glycerol, at 37°C Mus musculus