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EC Number
Metals/Ions
Commentary
Reference
3.4.24.27
Ca2+
-
683276
,
683691
3.4.24.27
Zn2+
-
683691
3.4.24.27
Co2+
2fold activation at 0.4 mM
650286
3.4.24.27
NaSCN
3fold increase in catalytic activity of thermolysin when the NaSCN concentration is increased to 1 M, but decrease in catalytic activity at higher concentrations of NaSCN
717283
3.4.24.27
Ca2+
4 calcium ions required for structural stability
651799
3.4.24.27
NaBr
4 M enhances activity 13-15 times
651789
3.4.24.27
NaCl
4 M enhances activity 13-15 times
651789
3.4.24.27
Na+
4 M Na+ stimulates the hydrolytic activity of wild type thermolysin about 13fold
712532
3.4.24.27
Zn2+
a single catalytic Zn2+ ion is essential for hydrolytic activity
711460
3.4.24.27
Zn2+
a zinc metalloprotease
683701
,
733524
,
733899
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