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Literature summary for 3.4.24.27 extracted from
- Effects of water-miscible solvents and polyhydroxy compounds on the structure and enzymatic activity of thermolysin (2006), J. Biotechnol., 127, 45-53.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dimethylformamide | - |
Bacillus thermoproteolyticus |  |
| DMSO | - |
Bacillus thermoproteolyticus | |
| Isopropanol | - |
Bacillus thermoproteolyticus | |
| n-Propanol | - |
Bacillus thermoproteolyticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Bacillus thermoproteolyticus | |
| Zn2+ | - |
Bacillus thermoproteolyticus | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| dimethylformamide | half-life is 6 min at 80°C, trehalose stabilizes the enzyme in DMF | Bacillus thermoproteolyticus |
| DMSO | half-life is over 20 min at 80°C | Bacillus thermoproteolyticus |
| isopropanol | half-life is 4 min at 80°C, stabilizes the enzyme | Bacillus thermoproteolyticus |
| additional information | solvents cause mixed inhibition of thermolysin, kinetic and structural studies, overview | Bacillus thermoproteolyticus |
| n-propanol | half-life is 3 min at 80°C, glycerol stabilizes the enzyme | Bacillus thermoproteolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thermoproteolyticus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bacillus thermoproteolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
thermoinactivation of thermolysin at 80°C in 50% of solvents, half-lives of 3-20 min, with the increase in solvent hydrophobicity, thermal stability of the enzyme decreases, overview | Bacillus thermoproteolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Bacillus thermoproteolyticus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | organic solvent inhibition kinetics, overview | Bacillus thermoproteolyticus | |
| 0.18 | - |
n-Propanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 0.25 | - |
Isopropanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 7.63 | - |
dimethylformamide | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 7.7 | - |
DMSO | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
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