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Literature summary for 3.4.24.27 extracted from

  • Kuzuya, K.; Inouye, K.
    Effects of cobalt-substitution of the active zinc ion in thermolysin on its activity and active-site microenvironment (2001), J. Biochem., 130, 783-788.
    View publication on PubMed
Search for more literature for 3.4.24.27

Crystallization (Commentary)

Crystallization (Comment) Organism
three-times crystallized preparation, Lot T5CB491 from Daiwa Kasei, Osaka Bacillus thermoproteolyticus

Inhibitors

Inhibitors Comment Organism Structure
Zincov competitive inhibitor Bacillus thermoproteolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ active zinc ion in thermolysin can be substituted by Co2+, doubles activity Bacillus thermoproteolyticus
NaBr 4 M enhances activity 13-15 times Bacillus thermoproteolyticus
NaCl 4 M enhances activity 13-15 times Bacillus thermoproteolyticus
Zn2+ active zinc ion in thermolysin Bacillus thermoproteolyticus

Organism

Organism UniProt Comment Textmining
Bacillus thermoproteolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
commercial preparation, used without further purification Bacillus thermoproteolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide + H2O
-
 Bacillus thermoproteolyticus ?
-
 r
Nalpha-benzyloxycarbonyl-L-aspartyl-L-phenylalanine methyl ester + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Bacillus thermoproteolyticus

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5
-
 Bacillus thermoproteolyticus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000012
-
 Zincov pH 7.5, 25°C, cobalt-substituted thermolysin Bacillus thermoproteolyticus
0.00023
-
 Zincov pH 7.5, 25°C Bacillus thermoproteolyticus