EC Number |
Metals/Ions |
Reference |
---|
3.4.24.27 | Ca2+ |
required |
717283, 717396 |
3.4.24.27 | Ca2+ |
required for stability |
683190 |
3.4.24.27 | Ca2+ |
stablizes the enzyme |
734109 |
3.4.24.27 | Ca2+ |
there are two adjacent calcium ions seemingly firmly bound inside the surface of the molecule by chelation to five acidic groups: Asp138, Glu177, Asp185, Glu190 and Asp191, two additional calcium binding sites are at exposed surface regions, one chelated by Asp57 and possibly also by Asp59 and the other chelated by Asp200 |
31146 |
3.4.24.27 | Ca2+ |
thermolysin consists of four Ca2+ ligand ions necessary for stability |
712856 |
3.4.24.27 | Ca2+ |
thermolysin has four calcium ions responsible for its thermostability |
711724 |
3.4.24.27 | Ca2+ |
TLN contains one zinc ion and four calcium ions, and these ions contribute to enzymatic catalysis and structural stability |
717113 |
3.4.24.27 | CaCl2 |
required |
734411 |
3.4.24.27 | Co2+ |
2fold activation at 0.4 mM |
650286 |
3.4.24.27 | Co2+ |
active zinc ion in thermolysin can be substituted by Co2+, doubles activity |
651789 |