EC Number   |
KM Value [mM] |
KM Value Maximum [mM]  |
Substrate |
Reference |
|---|
   3.6.1.17 | 0.003 |
- |
5',5''-diadenosine tetraphosphate |
mutant P133A, pH 7.5, 22°C |
733450 |
| 3.6.1.17 | 0.0041 |
- |
Ap4A |
mutant enzyme H38K |
656173 |
| 3.6.1.17 | 0.005 |
- |
diadenosine 5',5''-P1,P4-tetraphosphate |
presence of 5.0 mM MnCl2, pH 7.5, 22°C |
733346 |
| 3.6.1.17 | 0.005 |
- |
P1,P4-bis(5'-adenosyl) tetraphosphate |
- |
209901 |
| 3.6.1.17 | 0.005 |
- |
P1,P4-bis(5'-guanosyl) tetraphosphate |
- |
209894, 209896 |
| 3.6.1.17 | 0.0053 |
- |
Ap4A |
mutant enzyme E52Q |
656173 |
| 3.6.1.17 | 0.0058 |
- |
Ap4A |
mutant enzyme E103Q |
656173 |
| 3.6.1.17 | 0.0058 |
- |
Ap4A |
mutant enzyme H38G |
656173 |
| 3.6.1.17 | 0.007 |
- |
Ap4A |
pH 7.8, 25°C |
654817 |
| 3.6.1.17 | 0.007 |
- |
diadenosine 5',5''''-P1,P4-tetraphosphate |
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C |
719339 |
