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Information on EC 3.4.22.50 - V-cath endopeptidase for references in articles please use BRENDA:EC3.4.22.50
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EC Tree
The enzyme appears in viruses and cellular organisms
Reaction Schemes
endopeptidase of broad specificity, hydrolyzing substrates of both cathepsin L and cathepsin B
Synonyms
cathepsin, v-cath, viral cysteine protease, v-cathepsin, acnpv protease, viral cathepsin,
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baculovirus cathepsin
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nucleopolyhedrosis virus protease
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cathepsin
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V-cath
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viral cysteine protease
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viral cysteine protease
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endopeptidase of broad specificity, hydrolyzing substrates of both cathepsin L and cathepsin B
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hydrolysis of peptide bond
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-
-
-
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N-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
-
-
?
N-benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
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-
-
?
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
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-
-
?
N-benzyloxycarbonyl-Val-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Val-Arg + 7-amino-4-methylcoumarin
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-
-
?
N-benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
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-
-
?
azocasein + H2O
?
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?
azocasein + H2O
?
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-
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?
azocasein + H2O
?
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-
-
?
Azocoll + H2O
?
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-
-
?
Azocoll + H2O
?
-
-
-
-
?
Azocoll + H2O
?
-
-
-
-
?
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antipain
0.00005 mM, complete inhibition
chicken cystatin
0.0001 mM, complete inhibition
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iodoacetate
0.05 mM, 80% inhibition
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
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10 microM
E64
strong inhibition
E64
0.0001 mM, complete inhibition
E64
cysteine protease specific inhibitor
leupeptin
strong inhibition
leupeptin
0.00005 mM, complete inhibition
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Autographa californica multiple nucleopolyhedrovirus chitinase
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a proposed folding chaperone for the progenitor of V-CATH
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Urea
3 M, enhances activity
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0.003
Z-Arg-Arg-7-amido-4-methylcoumarin
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0.022
Z-Leu-Arg 7-amido-4-methylcoumarin
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0.039
Z-Phe-Arg 7-amido-4-methylcoumarin
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0.043
Z-Val-Arg-7-amido-4-methylcoumarin
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0.058
Z-Val-Val-Arg 7-amido-4-methylcoumarin
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0.73
Z-Arg-Arg-7-amido-4-methylcoumarin
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0.73
Z-Leu-Arg 7-amido-4-methylcoumarin
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0.95
Z-Phe-Arg 7-amido-4-methylcoumarin
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0.94
Z-Val-Arg-7-amido-4-methylcoumarin
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0.51
Z-Val-Val-Arg 7-amido-4-methylcoumarin
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5 - 5.5
bell-shaped profile, hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin and Z-Arg-Arg-7-amino-4-methylcoumarin
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3 - 7.5
hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin
4 - 7.5
hydrolysis of Z-Arg-Arg-7-amino-4-methylcoumarin
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SwissProt
brenda
CpGV-M1
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-
brenda
CpGV-M1
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-
brenda
-
SwissProt
brenda
baculovirus
SwissProt
brenda
in conjunction with chitinase, the enzyme promotes liquefaction of the host in the latter stages of infection
SwissProt
brenda
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-
brenda
baculovirus, enzyme may aid in the degradation of host proteins as a source of amino acids for viral protein production
SwissProt
brenda
-
SwissProt
brenda
sruce budworm virus
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-
brenda
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malfunction
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the absence of v-cathepsin results in poor melanization and liquefaction of Cydia pomonella larvae
physiological function
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v-cathepsin is involved in the degradation of larval tissues of Cydia pomonella
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CATV_NPVAC
323
0
36938
Swiss-Prot
CATV_NPVAH
337
0
37937
Swiss-Prot
CATV_NPVAP
324
0
36770
Swiss-Prot
CATV_NPVBM
323
0
36922
Swiss-Prot
CATV_NPVBS
331
0
37744
Swiss-Prot
CATV_NPVCD
324
1
36849
Swiss-Prot
CATV_NPVCF
324
0
36682
Swiss-Prot
CATV_NPVEP
323
0
36835
Swiss-Prot
CATV_NPVHC
324
0
36966
Swiss-Prot
CATV_NPVHZ
367
0
42203
Swiss-Prot
CATV_NPVLD
356
0
39770
Swiss-Prot
CATV_NPVMC
337
0
37890
Swiss-Prot
CATV_NPVOP
324
0
36982
Swiss-Prot
CATV_NPVR1
323
0
36965
Swiss-Prot
CATV_NPVSE
337
0
38387
Swiss-Prot
CATV_NPVST
337
0
38098
Swiss-Prot
CATV_GVCPM
333
0
37434
Swiss-Prot
CATV_GVXN
346
1
38819
Swiss-Prot
A0A098EAK7_9ZZZZ
676
1
75514
TrEMBL
G0QL92_ICHMG
Ichthyophthirius multifiliis (strain G5)
620
0
70895
TrEMBL
L7FM76_ENTIV
762
0
83937
TrEMBL
G0QU95_ICHMG
Ichthyophthirius multifiliis (strain G5)
250
0
28617
TrEMBL
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37000
x * 37000, deduced from nucleotide sequence
27500
x * 27500, 35500 Da and 32000 Da species are probaly precursors of the mature enzyme, immunoblot
27500
x * 27500, mature enzyme, calculated from amino acid sequence
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?
x * 27500, 35500 Da and 32000 Da species are probaly precursors of the mature enzyme, immunoblot; x * 37000, deduced from nucleotide sequence
?
x * 27500, mature enzyme, calculated from amino acid sequence
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glycoprotein
N-glycosylation may occur at Asn158
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additional information
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in this study a cysteine protease-deleted Bombyx mori multiple nucleopolyhedrovirus (CPD-BmMNPV) bacmid using the lambda recombination system is constructed. The protease activities of Bombyx mori cells and silkworm larvae infected with this CPD-BmMNPV bacmid are reduced by 94% and 85%, respectively. By using this system, a GFP(uv)-beta1,3-N-acetylglucosaminyltransferase 2 (GFP(uv)-beta3GnT2) fusion protein is successfully expressed in silkworm larvae with less protein degradation and without larvae liquefaction
additional information
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v-cath-deletion mutant, chloramphenicol acetyl transferase gene replaced the cathepsin gene
additional information
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v-cath-deletion mutant, chloramphenicol acetyl transferase gene replaced the cathepsin gene
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Ni-agarose column chromatography
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expressed in Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D
expression in Escherichia coli
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expression in Sf9 insect cells
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agriculture
the introduction of the v-cath gene into the genome of Anticarsia gemmatalis multiple nucleopolyhedrovirus improves its insecticidal activity against Anticarsia gemmatalis larvae
biotechnology
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this CPD-BmMNPV bacmid system provides rapid protein production in silkworms and can be used for the production of recombinant eukaryotic proteins without proteolytic degradation
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Ohkawa, T.; Majima, K.; Maeda, S.
A cysteine protease encoded by the baculovirus Bombyx mori nuclear polyhedrosis virus
J. Virol.
68
6619-6625
1994
Bombyx mori nucleopolyhedrovirus (P41721)
brenda
Slack, J.M.; Kuzio, J.; Faulkner, P.
Characterization of v-cath, a cathepsin L-like proteinase expressed by the baculovirus Autographa californica multiple nuclear polyhedrosis virus
J. Gen. Virol.
76
1091-1098
1995
Autographa californica nucleopolyhedrovirus (P25783)
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brenda
Bromme, D.; Okamoto, K.
The baculovirus cysteine protease has a cathepsin B-like S2-subsite specificity
Biol. Chem. Hoppe-Seyler
376
611-615
1995
Autographa californica nucleopolyhedrovirus, Autographa californica nucleopolyhedrovirus (P25783)
brenda
Hill, J.E.; Kuzio, J.; Faulkner, P.
Identification and characterization of the v-cath gene of the baculovirus, CfMNPV
Biochim. Biophys. Acta
1264
275-278
1995
Choristoneura fumiferana multiple nucleopolyhedrovirus
brenda
Hawtin, R.E.; Zarkowska, T.; Arnold, K.; Thomas, C.J.; Gooday, G.W.; King, L.A.; Kuzio, J.A.; Possee, R.D.
Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes
Virology
238
243-253
1997
Autographa californica nucleopolyhedrovirus, Autographa californica nucleopolyhedrovirus (P25783)
brenda
Hiyoshi, M.; Kageshima, A.; Kato, T.; Park, E.Y.
Construction of a cysteine protease deficient Bombyx mori multiple nucleopolyhedrovirus bacmid and its application to improve expression of a fusion protein
J. Virol. Methods
144
91-97
2007
Bombyx mori nucleopolyhedrovirus
brenda
Hilton, S.; Kemp, E.; Keane, G.; Winstanley, D.
A bacmid approach to the genetic manipulation of granuloviruses
J. Virol. Methods
152
56-62
2008
Cydia pomonella granulovirus, Cydia pomonella granulovirus CpGV-M1
brenda
Hodgson, J.J.; Arif, B.M.; Krell, P.J.
Role of interactions between Autographa californica multiple nucleopolyhedrovirus procathepsin and chitinase chitin-binding or active-site domains in viral cathepsin processing
J. Virol.
87
3471-3483
2013
Autographa californica multiple nucleopolyhedrovirus, Autographa californica multiple nucleopolyhedrovirus (P25783)
brenda
Lima, A.A.; Aragao, C.W.; de Castro, M.E.; Oliveira, J.V.; Sosa Gomez, D.R.; Ribeiro, B.M.
A recombinant Anticarsia gemmatalis MNPV harboring chiA and v-cath genes from Choristoneura fumiferana defective NPV induce host liquefaction and increased insecticidal activity
PLoS ONE
8
e74592
2013
Choristoneura fumiferana multiple nucleopolyhedrovirus (P41715)
brenda
Ishimwe, E.; Hodgson, J.J.; Passarelli, A.L.
Expression of the Cydia pomonella granulovirus matrix metalloprotease enhances Autographa californica multiple nucleopolyhedrovirus virulence and can partially substitute for viral cathepsin
Virology
481
166-178
2015
Autographa californica multiple nucleopolyhedrovirus, Autographa californica multiple nucleopolyhedrovirus (P25783)
brenda
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3.4.22.50 V-cath endopeptidase
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