Literature summary for 3.4.22.50 extracted from

Hiyoshi, M.; Kageshima, A.; Kato, T.; Park, E.Y.
Construction of a cysteine protease deficient Bombyx mori multiple nucleopolyhedrovirus bacmid and its application to improve expression of a fusion protein (2007), J. Virol. Methods, 144, 91-97.

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Application

Application	Comment	Organism
biotechnology	this CPD-BmMNPV bacmid system provides rapid protein production in silkworms and can be used for the production of recombinant eukaryotic proteins without proteolytic degradation	Bombyx mori nucleopolyhedrovirus

Protein Variants

Protein Variants	Comment	Organism
additional information	in this study a cysteine protease-deleted Bombyx mori multiple nucleopolyhedrovirus (CPD-BmMNPV) bacmid using the lambda recombination system is constructed. The protease activities of Bombyx mori cells and silkworm larvae infected with this CPD-BmMNPV bacmid are reduced by 94% and 85%, respectively. By using this system, a GFP(uv)-beta1,3-N-acetylglucosaminyltransferase 2 (GFP(uv)-beta3GnT2) fusion protein is successfully expressed in silkworm larvae with less protein degradation and without larvae liquefaction	Bombyx mori nucleopolyhedrovirus

Organism

Organism	UniProt	Comment	Textmining
Bombyx mori nucleopolyhedrovirus	-	-	-

Synonyms

Synonyms	Comment	Organism
cysteine protease	-	Bombyx mori nucleopolyhedrovirus
V-cath	-	Bombyx mori nucleopolyhedrovirus

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Release 2023.1 (January 2023)