Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.50 extracted from

  • Bromme, D.; Okamoto, K.
    The baculovirus cysteine protease has a cathepsin B-like S2-subsite specificity (1995), Biol. Chem. Hoppe-Seyler, 376, 611-615.
    View publication on PubMed
Search for more literature for 3.4.22.50

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 insect cells Autographa californica nucleopolyhedrovirus

Inhibitors

Inhibitors Comment Organism Structure
antipain 0.00005 mM, complete inhibition Autographa californica nucleopolyhedrovirus
chicken cystatin 0.0001 mM, complete inhibition Autographa californica nucleopolyhedrovirus
E64 0.0001 mM, complete inhibition Autographa californica nucleopolyhedrovirus
iodoacetate 0.05 mM, 80% inhibition Autographa californica nucleopolyhedrovirus
leupeptin 0.00005 mM, complete inhibition Autographa californica nucleopolyhedrovirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 Z-Arg-Arg-7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
0.022
-
 Z-Leu-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
0.039
-
 Z-Phe-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
0.043
-
 Z-Val-Arg-7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
0.058
-
 Z-Val-Val-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus

Organism

Organism UniProt Comment Textmining
Autographa californica nucleopolyhedrovirus P25783
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Autographa californica nucleopolyhedrovirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Autographa californica nucleopolyhedrovirus N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Autographa californica nucleopolyhedrovirus N-benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Autographa californica nucleopolyhedrovirus N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzyloxycarbonyl-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Autographa californica nucleopolyhedrovirus N-benzyloxycarbonyl-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Autographa californica nucleopolyhedrovirus N-benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
Z-Leu-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus Z-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.51
-
 Z-Val-Val-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
0.73
-
 Z-Arg-Arg-7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
0.73
-
 Z-Leu-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
0.94
-
 Z-Val-Arg-7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus
0.95
-
 Z-Phe-Arg 7-amido-4-methylcoumarin
-
 Autographa californica nucleopolyhedrovirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5 bell-shaped profile, hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin and Z-Arg-Arg-7-amino-4-methylcoumarin Autographa californica nucleopolyhedrovirus

pH Range

pH Minimum pH Maximum Comment Organism
3 7.5 hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin Autographa californica nucleopolyhedrovirus
4 7.5 hydrolysis of Z-Arg-Arg-7-amino-4-methylcoumarin Autographa californica nucleopolyhedrovirus