activity in expanding fifth leaves is increased 8- to 20fold by growth at high temperatures(35°C day/30°C night) compared to cool temperatures (15°C/10°C). Several days of high temperature are required for full induction of activity. No induction occurs in leaves which completed expansion in cool conditions before exposure to high temperature
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expression is induced by methyl jasmonate. Infestation by Rhopalosiphum padi and Metopolophium dirhodum results in higher expression in the doubled haploid line 5172-28:4. None of the aphide-plant combinations displays increased gramine levels, and the increased abundance of transcript in aphid-infested DH28:4 does not lead to higher amounts of NMT protein or NMT enzyme activity