Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | gramine levels, but not NMT expression, is increased in barley due to drought and abscisic acid treatment | Hordeum vulgare |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Hordeum vulgare subsp. vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
3-aminomethylindole | pH 7.5, 30°C | Hordeum vulgare subsp. vulgare | |
0.148 | - |
S-adenosyl-L-methionine | pH 7.5, 30°C | Hordeum vulgare subsp. vulgare | |
0.184 | - |
N-methyl-3-aminomethylindole | pH 7.5, 30°C | Hordeum vulgare subsp. vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE | Hordeum vulgare subsp. vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Hordeum vulgare subsp. vulgare | Q96565 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + 3-aminomethylindole | - |
Hordeum vulgare subsp. vulgare | 2 S-adenosyl-L-homocysteine + gramine | overall reaction, two successive N-methylation reactions | ? | |
2 S-adenosyl-L-methionine + N-methyl-3-aminomethylindole | - |
Hordeum vulgare subsp. vulgare | 2 S-adenosyl-L-homocysteine + (1H-indol-3-yl)-N,N-dimethylmethanamine | - |
? | |
additional information | no substrate: caffeic acid | Hordeum vulgare subsp. vulgare | ? | - |
? | |
S-adenosyl-L-methionine + 3-aminomethylindole | - |
Hordeum vulgare subsp. vulgare | S-adenosyl-L-homocysteine + N-methyl-3-aminomethylindole | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Hordeum vulgare subsp. vulgare |
Synonyms | Comment | Organism |
---|---|---|
NMT | - |
Hordeum vulgare |
Organism | Comment | Expression |
---|---|---|
Hordeum vulgare | expression is induced by methyl jasmonate. Infestation by Rhopalosiphum padi and Metopolophium dirhodum results in higher expression in the doubled haploid line 5172-28:4. None of the aphide-plant combinations displays increased gramine levels, and the increased abundance of transcript in aphid-infested DH28:4 does not lead to higher amounts of NMT protein or NMT enzyme activity | up |