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Information on EC 1.1.99.27 - (R)-pantolactone dehydrogenase (flavin) for references in articles please use BRENDA:EC1.1.99.27
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IUBMB Comments High specificity for (R)-pantolactone. Phenazine methosulfate (PMS) can act as acceptor. The enzyme has been studied in the bacterium Nocardia asteroides and shown to be membrane-bound and induced by 1,2-propanediol. The FMN cofactor is non-covalently bound.
The expected taxonomic range for this enzyme is: Nocardia asteroides
Synonyms
(R)-Pantoyllactone dehydrogenase (flavin), 2-Dehydropantolactone reductase (flavin), 2-Dehydropantoyl-lactone reductase (flavin), dehydrogenase, L-(+)-pantoyllactone,
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(R)-Pantoyllactone dehydrogenase (flavin)
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2-Dehydropantolactone reductase (flavin)
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2-Dehydropantoyl-lactone reductase (flavin)
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dehydrogenase, L-(+)-pantoyllactone
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(R)-Pantolactone + acceptor = 2-dehydropantolactone + reduced acceptor
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(R)-pantolactone:acceptor oxidoreductase (flavin-containing)
High specificity for (R)-pantolactone. Phenazine methosulfate (PMS) can act as acceptor. The enzyme has been studied in the bacterium Nocardia asteroides and shown to be membrane-bound and induced by 1,2-propanediol. The FMN cofactor is non-covalently bound.
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(R)-Pantolactone + (Fe(CN)6)3-
2-Dehydropantonolactone + Fe(CN)64-
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(R)-Pantolactone + 2,6-dichlorophenol-indophenol
2-Dehydropantonolactone + reduced 2,6-dichlorophenol-indophenol
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(R)-Pantolactone + indonitrotetrazolium
2-Dehydropantonolactone + reduced indonitrotetrazolium
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(R)-Pantolactone + medola blue
2-Dehydropantonolactone + reduced medola blue
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(R)-Pantolactone + nitrotetrazolium blue
2-Dehydropantonolactone + reduced nitrotetrazolium blue
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(R)-Pantolactone + phenazine methosulfate
2-Dehydropantonolactone + reduced phenazine methosulfate
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highly specific for (R)-pantolactone
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additional information
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1,2-propanediol induces the enzyme
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additional information
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1,2-propanediol induces the enzyme
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FMN
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dependent on, noncovalently bound
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2-Dehydropantonolactone
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20 mM
5,5'-dithiobis(2-nitrobenzoate)
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1 mM, 47% inhibition
Atebrin
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1 mM, 32.6% inhibition
Barbital
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1 mM, 54.5% inhibition
CoCl2
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1 mM, 24.4% inhibition
Diphenylhydantoin
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1 mM, 22.1% inhibition
iodoacetate
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1 mM, 17.4% inhibition
MnCl2
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1 mM, 86.2% inhibition
NaAsO2
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1 mM, 41% inhibition
NaCN
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1 mM, 51.9% inhibition
NaF
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1 mM, 27.3% inhibition
NaN3
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1 mM, 9.1% inhibition
NEM
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1 mM, 33.8% inhibition
NH2OH*HCl
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1 mM, 71.9% inhibition
PCMB
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1 mM, 41.0% inhibition
riboflavin
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1 mM, 74.9% inhibition
Semicarbazide
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1 mM, 23.1% inhibition
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Brij 35
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activates, maximal stimulation at 1.0%
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26.8
(R)-pantolactone
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25 - 45
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about 20% of maximal activity at 25°C and 45°C
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brenda
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bound
brenda
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H7CGG6_RHOER
392
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41612
TrEMBL
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42000
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x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol
600000
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above 600000 Da, HPLC gel filtration
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x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol
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30
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pH 9.0-10.0, 30 min, stable
35
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pH 7.0, 10 min, stable below
45
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pH 7.0, 10 min, 43.1% loss of activity
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some stabilization by glycerol and ethylene glycol
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4°C, pH 7.4, 0.1 U/ml enzyme, 0.01 M Tris-HCl, 0.1 mM dithiothreitol, 0.5% Brij35, 2.9% loss of activity after 2 weeks
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Kataoka, M.; Shimizu, S.; Yamada, H.
Purification and characterization of a novel FMN-dependent enzyme. Membrane-bound L-(+)-pantoyl lactone dehydrogenase from Nocardia asteroides
Eur. J. Biochem.
204
799-806
1992
Nocardia asteroides
brenda
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