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Information on EC 1.1.99.27 - (R)-pantolactone dehydrogenase (flavin)

for references in articles please use BRENDA:EC1.1.99.27

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The expected taxonomic range for this enzyme is: Nocardia asteroides

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1.1.99.27

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(R)-pantolactone dehydrogenase (flavin)

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(R)-Pantolactone + acceptor = 2-dehydropantolactone + reduced acceptor

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oxidation

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reduction

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(R)-pantolactone:acceptor oxidoreductase (flavin-containing)

High specificity for (R)-pantolactone. Phenazine methosulfate (PMS) can act as acceptor. The enzyme has been studied in the bacterium Nocardia asteroides and shown to be membrane-bound and induced by 1,2-propanediol. The FMN cofactor is non-covalently bound.

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Show Synonyms (4 entries)

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140879-14-7

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Nocardia asteroides

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Manually annotated by BRENDA team

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(R)-Pantolactone + (Fe(CN)6)3-

2-Dehydropantonolactone + Fe(CN)64-

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Nocardia asteroides

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(R)-Pantolactone + 2,6-dichlorophenol-indophenol

2-Dehydropantonolactone + reduced 2,6-dichlorophenol-indophenol

show the reaction diagram

Nocardia asteroides

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(R)-Pantolactone + indonitrotetrazolium

2-Dehydropantonolactone + reduced indonitrotetrazolium

show the reaction diagram

Nocardia asteroides

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(R)-Pantolactone + medola blue

2-Dehydropantonolactone + reduced medola blue

show the reaction diagram

Nocardia asteroides

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(R)-Pantolactone + nitrotetrazolium blue

2-Dehydropantonolactone + reduced nitrotetrazolium blue

show the reaction diagram

Nocardia asteroides

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(R)-Pantolactone + phenazine methosulfate

2-Dehydropantonolactone + reduced phenazine methosulfate

show the reaction diagram

Nocardia asteroides

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highly specific for (R)-pantolactone

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additional information

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Nocardia asteroides

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1,2-propanediol induces the enzyme

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additional information

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Nocardia asteroides

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1,2-propanediol induces the enzyme

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FMN

Nocardia asteroides

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dependent on, noncovalently bound

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(R)-pantolactone

Nocardia asteroides

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2-Dehydropantonolactone

Nocardia asteroides

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20 mM

5,5'-dithiobis(2-nitrobenzoate)

Nocardia asteroides

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1 mM, 47% inhibition

Atebrin

Nocardia asteroides

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1 mM, 32.6% inhibition

Barbital

Nocardia asteroides

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1 mM, 54.5% inhibition

CoCl2

Nocardia asteroides

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1 mM, 24.4% inhibition

Diphenylhydantoin

Nocardia asteroides

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1 mM, 22.1% inhibition

iodoacetate

Nocardia asteroides

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1 mM, 17.4% inhibition

MnCl2

Nocardia asteroides

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1 mM, 86.2% inhibition

NaAsO2

Nocardia asteroides

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1 mM, 41% inhibition

NaCN

Nocardia asteroides

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1 mM, 51.9% inhibition

NaF

Nocardia asteroides

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1 mM, 27.3% inhibition

NaN3

Nocardia asteroides

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1 mM, 9.1% inhibition

NEM

Nocardia asteroides

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1 mM, 33.8% inhibition

NH2OH*HCl

Nocardia asteroides

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1 mM, 71.9% inhibition

PCMB

Nocardia asteroides

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1 mM, 41.0% inhibition

riboflavin

Nocardia asteroides

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1 mM, 74.9% inhibition

Semicarbazide

Nocardia asteroides

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1 mM, 23.1% inhibition

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Brij 35

Nocardia asteroides

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activates, maximal stimulation at 1.0%

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26.8

(R)-pantolactone

Nocardia asteroides

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9 - 9.5

Nocardia asteroides

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40

Nocardia asteroides

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25 - 45

Nocardia asteroides

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about 20% of maximal activity at 25°C and 45°C

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Nocardia asteroides

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bound

Manually annotated by BRENDA team

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42000

Nocardia asteroides

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x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol

600000

Nocardia asteroides

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above 600000 Da, HPLC gel filtration

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?

Nocardia asteroides

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x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol

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no glycoprotein

Nocardia asteroides

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30

Nocardia asteroides

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pH 9.0-10.0, 30 min, stable

35

Nocardia asteroides

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pH 7.0, 10 min, stable below

45

Nocardia asteroides

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pH 7.0, 10 min, 43.1% loss of activity

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Brij35 stabilizes

Nocardia asteroides

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some stabilization by glycerol and ethylene glycol

Nocardia asteroides

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4°C, pH 7.4, 0.1 U/ml enzyme, 0.01 M Tris-HCl, 0.1 mM dithiothreitol, 0.5% Brij35, 2.9% loss of activity after 2 weeks

Nocardia asteroides

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Nocardia asteroides

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Show AA Sequence (1 entry)

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Show References (1 entry)

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NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)