We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments High specificity for (R)-pantolactone. Phenazine methosulfate (PMS) can act as acceptor. The enzyme has been studied in the bacterium Nocardia asteroides and shown to be membrane-bound and induced by 1,2-propanediol. The FMN cofactor is non-covalently bound.
The enzyme appears in viruses and cellular organisms
Synonyms
(R)-Pantoyllactone dehydrogenase (flavin), 2-Dehydropantolactone reductase (flavin), 2-Dehydropantoyl-lactone reductase (flavin), dehydrogenase, L-(+)-pantoyllactone,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)-Pantoyllactone dehydrogenase (flavin)
-
-
-
-
2-Dehydropantolactone reductase (flavin)
-
-
-
-
2-Dehydropantoyl-lactone reductase (flavin)
-
-
-
-
dehydrogenase, L-(+)-pantoyllactone
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)-Pantolactone + acceptor = 2-dehydropantolactone + reduced acceptor
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)-pantolactone:acceptor oxidoreductase (flavin-containing)
High specificity for (R)-pantolactone. Phenazine methosulfate (PMS) can act as acceptor. The enzyme has been studied in the bacterium Nocardia asteroides and shown to be membrane-bound and induced by 1,2-propanediol. The FMN cofactor is non-covalently bound.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)-Pantolactone + (Fe(CN)6)3-
2-Dehydropantonolactone + Fe(CN)64-
-
-
-
-
?
(R)-Pantolactone + 2,6-dichlorophenol-indophenol
2-Dehydropantonolactone + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
(R)-Pantolactone + indonitrotetrazolium
2-Dehydropantonolactone + reduced indonitrotetrazolium
-
-
-
-
?
(R)-Pantolactone + medola blue
2-Dehydropantonolactone + reduced medola blue
-
-
-
-
?
(R)-Pantolactone + nitrotetrazolium blue
2-Dehydropantonolactone + reduced nitrotetrazolium blue
-
-
-
-
?
(R)-Pantolactone + phenazine methosulfate
2-Dehydropantonolactone + reduced phenazine methosulfate
-
highly specific for (R)-pantolactone
-
-
?
additional information
?
-
-
1,2-propanediol induces the enzyme
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
?
-
-
1,2-propanediol induces the enzyme
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
FMN
-
dependent on, noncovalently bound
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-Dehydropantonolactone
-
20 mM
5,5'-dithiobis(2-nitrobenzoate)
-
1 mM, 47% inhibition
Atebrin
-
1 mM, 32.6% inhibition
Barbital
-
1 mM, 54.5% inhibition
CoCl2
-
1 mM, 24.4% inhibition
Diphenylhydantoin
-
1 mM, 22.1% inhibition
iodoacetate
-
1 mM, 17.4% inhibition
MnCl2
-
1 mM, 86.2% inhibition
NaAsO2
-
1 mM, 41% inhibition
NaCN
-
1 mM, 51.9% inhibition
NaF
-
1 mM, 27.3% inhibition
NaN3
-
1 mM, 9.1% inhibition
NEM
-
1 mM, 33.8% inhibition
NH2OH*HCl
-
1 mM, 71.9% inhibition
PCMB
-
1 mM, 41.0% inhibition
riboflavin
-
1 mM, 74.9% inhibition
Semicarbazide
-
1 mM, 23.1% inhibition
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Brij 35
-
activates, maximal stimulation at 1.0%
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
26.8
(R)-pantolactone
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
25 - 45
-
about 20% of maximal activity at 25°C and 45°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
bound
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
H7CGG6_RHOER
392
0
41612
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
42000
-
x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol
600000
-
above 600000 Da, HPLC gel filtration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
30
-
pH 9.0-10.0, 30 min, stable
35
-
pH 7.0, 10 min, stable below
45
-
pH 7.0, 10 min, 43.1% loss of activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
some stabilization by glycerol and ethylene glycol
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4°C, pH 7.4, 0.1 U/ml enzyme, 0.01 M Tris-HCl, 0.1 mM dithiothreitol, 0.5% Brij35, 2.9% loss of activity after 2 weeks
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Kataoka, M.; Shimizu, S.; Yamada, H.
Purification and characterization of a novel FMN-dependent enzyme. Membrane-bound L-(+)-pantoyl lactone dehydrogenase from Nocardia asteroides
Eur. J. Biochem.
204
799-806
1992
Nocardia asteroides
brenda
Select items on the left to see more content.
html completed