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show all sequences of 1.1.99.27

Purification and characterization of a novel FMN-dependent enzyme. Membrane-bound L-(+)-pantoyl lactone dehydrogenase from Nocardia asteroides

Kataoka, M.; Shimizu, S.; Yamada, H.; Eur. J. Biochem. 204, 799-806 (1992)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Brij 35
activates, maximal stimulation at 1.0%
Nocardia asteroides
General Stability
General Stability
Organism
Brij35 stabilizes
Nocardia asteroides
some stabilization by glycerol and ethylene glycol
Nocardia asteroides
Inhibitors
Inhibitors
Commentary
Organism
Structure
(R)-pantolactone
-
Nocardia asteroides
2-Dehydropantonolactone
20 mM
Nocardia asteroides
5,5'-dithiobis(2-nitrobenzoate)
1 mM, 47% inhibition
Nocardia asteroides
Atebrin
1 mM, 32.6% inhibition
Nocardia asteroides
Barbital
1 mM, 54.5% inhibition
Nocardia asteroides
CoCl2
1 mM, 24.4% inhibition
Nocardia asteroides
Diphenylhydantoin
1 mM, 22.1% inhibition
Nocardia asteroides
iodoacetate
1 mM, 17.4% inhibition
Nocardia asteroides
MnCl2
1 mM, 86.2% inhibition
Nocardia asteroides
NaAsO2
1 mM, 41% inhibition
Nocardia asteroides
NaCN
1 mM, 51.9% inhibition
Nocardia asteroides
NaF
1 mM, 27.3% inhibition
Nocardia asteroides
NaN3
1 mM, 9.1% inhibition
Nocardia asteroides
NEM
1 mM, 33.8% inhibition
Nocardia asteroides
NH2OH*HCl
1 mM, 71.9% inhibition
Nocardia asteroides
PCMB
1 mM, 41.0% inhibition
Nocardia asteroides
riboflavin
1 mM, 74.9% inhibition
Nocardia asteroides
Semicarbazide
1 mM, 23.1% inhibition
Nocardia asteroides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
26.8
-
(R)-pantolactone
-
Nocardia asteroides
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound
Nocardia asteroides
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol
Nocardia asteroides
600000
-
above 600000 Da, HPLC gel filtration
Nocardia asteroides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Nocardia asteroides
1,2-propanediol induces the enzyme
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nocardia asteroides
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
no glycoprotein
-
Nocardia asteroides
Purification (Commentary)
Commentary
Organism
-
Nocardia asteroides
Storage Stability
Storage Stability
Organism
4°C, pH 7.4, 0.1 U/ml enzyme, 0.01 M Tris-HCl, 0.1 mM dithiothreitol, 0.5% Brij35, 2.9% loss of activity after 2 weeks
Nocardia asteroides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-Pantolactone + (Fe(CN)6)3-
-
10899
Nocardia asteroides
2-Dehydropantonolactone + Fe(CN)64-
-
-
-
-
(R)-Pantolactone + 2,6-dichlorophenol-indophenol
-
10899
Nocardia asteroides
2-Dehydropantonolactone + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
(R)-Pantolactone + indonitrotetrazolium
-
10899
Nocardia asteroides
2-Dehydropantonolactone + reduced indonitrotetrazolium
-
-
-
-
(R)-Pantolactone + medola blue
-
10899
Nocardia asteroides
2-Dehydropantonolactone + reduced medola blue
-
-
-
-
(R)-Pantolactone + nitrotetrazolium blue
-
10899
Nocardia asteroides
2-Dehydropantonolactone + reduced nitrotetrazolium blue
-
-
-
-
(R)-Pantolactone + phenazine methosulfate
highly specific for (R)-pantolactone
10899
Nocardia asteroides
2-Dehydropantonolactone + reduced phenazine methosulfate
-
-
-
-
additional information
1,2-propanediol induces the enzyme
10899
Nocardia asteroides
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol
Nocardia asteroides
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Nocardia asteroides
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
45
about 20% of maximal activity at 25°C and 45°C
Nocardia asteroides
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
pH 9.0-10.0, 30 min, stable
Nocardia asteroides
35
-
pH 7.0, 10 min, stable below
Nocardia asteroides
45
-
pH 7.0, 10 min, 43.1% loss of activity
Nocardia asteroides
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Nocardia asteroides
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
dependent on, noncovalently bound
Nocardia asteroides
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Brij 35
activates, maximal stimulation at 1.0%
Nocardia asteroides
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
dependent on, noncovalently bound
Nocardia asteroides
General Stability (protein specific)
General Stability
Organism
Brij35 stabilizes
Nocardia asteroides
some stabilization by glycerol and ethylene glycol
Nocardia asteroides
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(R)-pantolactone
-
Nocardia asteroides
2-Dehydropantonolactone
20 mM
Nocardia asteroides
5,5'-dithiobis(2-nitrobenzoate)
1 mM, 47% inhibition
Nocardia asteroides
Atebrin
1 mM, 32.6% inhibition
Nocardia asteroides
Barbital
1 mM, 54.5% inhibition
Nocardia asteroides
CoCl2
1 mM, 24.4% inhibition
Nocardia asteroides
Diphenylhydantoin
1 mM, 22.1% inhibition
Nocardia asteroides
iodoacetate
1 mM, 17.4% inhibition
Nocardia asteroides
MnCl2
1 mM, 86.2% inhibition
Nocardia asteroides
NaAsO2
1 mM, 41% inhibition
Nocardia asteroides
NaCN
1 mM, 51.9% inhibition
Nocardia asteroides
NaF
1 mM, 27.3% inhibition
Nocardia asteroides
NaN3
1 mM, 9.1% inhibition
Nocardia asteroides
NEM
1 mM, 33.8% inhibition
Nocardia asteroides
NH2OH*HCl
1 mM, 71.9% inhibition
Nocardia asteroides
PCMB
1 mM, 41.0% inhibition
Nocardia asteroides
riboflavin
1 mM, 74.9% inhibition
Nocardia asteroides
Semicarbazide
1 mM, 23.1% inhibition
Nocardia asteroides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
26.8
-
(R)-pantolactone
-
Nocardia asteroides
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound
Nocardia asteroides
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol
Nocardia asteroides
600000
-
above 600000 Da, HPLC gel filtration
Nocardia asteroides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Nocardia asteroides
1,2-propanediol induces the enzyme
?
-
-
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
no glycoprotein
-
Nocardia asteroides
Purification (Commentary) (protein specific)
Commentary
Organism
-
Nocardia asteroides
Storage Stability (protein specific)
Storage Stability
Organism
4°C, pH 7.4, 0.1 U/ml enzyme, 0.01 M Tris-HCl, 0.1 mM dithiothreitol, 0.5% Brij35, 2.9% loss of activity after 2 weeks
Nocardia asteroides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-Pantolactone + (Fe(CN)6)3-
-
10899
Nocardia asteroides
2-Dehydropantonolactone + Fe(CN)64-
-
-
-
-
(R)-Pantolactone + 2,6-dichlorophenol-indophenol
-
10899
Nocardia asteroides
2-Dehydropantonolactone + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
(R)-Pantolactone + indonitrotetrazolium
-
10899
Nocardia asteroides
2-Dehydropantonolactone + reduced indonitrotetrazolium
-
-
-
-
(R)-Pantolactone + medola blue
-
10899
Nocardia asteroides
2-Dehydropantonolactone + reduced medola blue
-
-
-
-
(R)-Pantolactone + nitrotetrazolium blue
-
10899
Nocardia asteroides
2-Dehydropantonolactone + reduced nitrotetrazolium blue
-
-
-
-
(R)-Pantolactone + phenazine methosulfate
highly specific for (R)-pantolactone
10899
Nocardia asteroides
2-Dehydropantonolactone + reduced phenazine methosulfate
-
-
-
-
additional information
1,2-propanediol induces the enzyme
10899
Nocardia asteroides
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol
Nocardia asteroides
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Nocardia asteroides
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
45
about 20% of maximal activity at 25°C and 45°C
Nocardia asteroides
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
pH 9.0-10.0, 30 min, stable
Nocardia asteroides
35
-
pH 7.0, 10 min, stable below
Nocardia asteroides
45
-
pH 7.0, 10 min, 43.1% loss of activity
Nocardia asteroides
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Nocardia asteroides
Other publictions for EC 1.1.99.27
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
10899
Kataoka
Purification and characterizat ...
Nocardia asteroides
Eur. J. Biochem.
204
799-806
1992
1
-
-
-
-
2
18
1
1
-
2
1
-
1
-
1
1
-
-
-
-
1
7
1
1
1
3
-
1
-
-
1
-
-
-
1
-
-
1
-
-
2
-
18
-
1
1
-
2
1
-
-
1
1
-
-
-
1
7
1
1
1
3
-
1
-
-
-
-
-
-
-
-
-