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EC 2.8.1.4 Details

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EC number

2.8.1.4

Accepted name

tRNA uracil 4-sulfurtransferase

Reaction

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster

Other name(s)

thiI (gene name), transfer ribonucleate sulfurtransferase (ambiguous), RNA sulfurtransferase (ambiguous), ribonucleate sulfurtransferase (ambiguous), transfer RNA sulfurtransferase (ambiguous), transfer RNA thiolase (ambiguous), L-cysteine:tRNA sulfurtransferase (incorrect), tRNA sulfurtransferase (ambiguous)

Systematic name

[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine:uracil in tRNA sulfurtransferase

CAS registry number

9055-57-6

Comment

The enzyme, found in bacteria and archaea, is activated by EC 2.8.1.7, cysteine desulfurase, which transfers a sulfur atom to an internal L-cysteine residue, forming a cysteine persulfide. The activated enzyme then transfers the sulfur to a uridine in a tRNA chain in a reaction that requires ATP. The enzyme from the bacterium Escherichia coli forms 4-thiouridine only at position 8 of tRNA. The enzyme also participates in the biosynthesis of the thiazole moiety of thiamine, but different domains are involved in the two processes.

History

created 1984, modified 2017

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.12.1 With NAD⁺ or NADP⁺ as acceptor

1.12.2 With a cytochrome as acceptor

1.12.5 With a quinone or similar compound as acceptor

1.12.7 With an iron-sulfur protein as acceptor

1.12.98 With other, known, physiological acceptors

1.12.99 With unknown physiological acceptors

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.13.1. undefined

1.13.11 With incorporation of two atoms of oxygen

1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

1.13.99 Miscellaneous

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.15 Acting on superoxide as acceptor

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.21.1 With NAD⁺ or NADP⁺ as acceptor

1.21.3 With oxygen as acceptor

1.21.4 With a disulfide as acceptor

1.21.98 With other, known, physiological acceptors

1.21.99 With unknown physiological acceptors

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.97 Other oxidoreductases

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)

2 Transferases

2.1 Transferring one-carbon groups

2.2 Transferring aldehyde or ketonic groups

2.3 Acyltransferases

2.4 Glycosyltransferases

2.5 Transferring alkyl or aryl groups, other than methyl groups

2.6 Transferring nitrogenous groups

2.7 Transferring phosphorus-containing groups

2.8 Transferring sulfur-containing groups

2.8.1 Sulfurtransferases

2.8.1.1 thiosulfate sulfurtransferase

2.8.1.2 3-mercaptopyruvate sulfurtransferase

2.8.1.3 thiosulfate—thiol sulfurtransferase

2.8.1.4 tRNA uracil 4-sulfurtransferase

2.8.1.5 thiosulfate—dithiol sulfurtransferase

2.8.1.6 biotin synthase

2.8.1.7 cysteine desulfurase

2.8.1.8 lipoyl synthase

2.8.1.9 molybdenum cofactor sulfurtransferase

2.8.1.10 thiazole synthase

2.8.1.11 molybdopterin synthase sulfurtransferase

2.8.1.12 molybdopterin synthase

2.8.1.13 tRNA-uridine 2-sulfurtransferase

2.8.1.14 tRNA-5-taurinomethyluridine 2-sulfurtransferase

2.8.1.15 tRNA-5-methyluridine⁵⁴ 2-sulfurtransferase

2.8.1.16 L-aspartate semialdehyde sulfurtransferase

2.8.2 Sulfotransferases

2.8.3 CoA-transferases

2.8.4 Transferring alkylthio groups

2.8.5 Thiosulfotransferases

2.9 Transferring selenium-containing groups

2.10 Transferring molybdenum- or tungsten-containing groups