EC Explorer

1.5.1.54

methylenetetrahydrofolate reductase (NADH)

5-methyltetrahydrofolate + NAD⁺ = 5,10-methylenetetrahydrofolate + NADH + H⁺

metF (gene name), 5,10-methylenetetrahydrofolic acid reductase (ambiguous), 5,10-CH₂-H₄folate reductase (ambiguous), methylenetetrahydrofolate (reduced riboflavin adenine dinucleotide) reductase, 5,10-methylenetetrahydrofolate reductase (ambiguous), methylenetetrahydrofolate reductase (ambiguous), N⁵,10-methylenetetrahydrofolate reductase (ambiguous), 5,10-methylenetetrahydropteroylglutamate reductase (ambiguous), N⁵,N¹⁰-methylenetetrahydrofolate reductase (ambiguous), methylenetetrahydrofolic acid reductase (ambiguous), 5-methyltetrahydrofolate:(acceptor) oxidoreductase (incorrect), 5,10-methylenetetrahydrofolate reductase (FADH₂) (ambiguous)

5-methyltetrahydrofolate:NAD⁺ oxidoreductase

71822-25-8

A flavoprotein (FAD). The enzyme, found in plants and some bacteria, catalyses the reversible conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate using NADH as the electron donor. It play an important role in folate metabolism by regulating the distribution of one-carbon moieties between cellular methylation reactions and nucleic acid synthesis. These proteins either contain a C-terminal domain that does not mediate allosteric regulation (as in plants) or lack this domain entirely (as in Escherichia coli). As a result, the plant enzymes are not inhibited by S-adenosyl-L-methionine, unlike other eukaryotic enzymes, and catalyse a reversible reaction. cf. EC 1.5.1.53, methylenetetrahydrofolate reductase (NADPH); EC 1.5.1.20, methylenetetrahydrofolate reductase [NAD(P)H]; and EC 1.5.7.1, methylenetetrahydrofolate reductase (ferredoxin).

created 2021