EC Explorer
[browse EC tree] - [search]
EC 1.5.3.25 Details
show Brenda entryEC number
1.5.3.25
Accepted name
fructosyl amine oxidase (glucosone-forming)
Reaction
an N-(1-deoxy-D-fructos-1-yl)amine + O2 + H2O = D-glucosone + an amine + H2O2 (overall reaction);;(1a) an N-(1-deoxy-D-fructos-1-yl)amine + O2 = a 2-[(3S,4R,5R)-3,4,5,6-tetrahydroxy-2-oxohexylidene]amine + H2O2;;(1b) a 2-[(3S,4R,5R)-3,4,5,6-tetrahydroxy-2-oxohexylidene]amine + H2O = D-glucosone + an amine (spontaneous)
Other name(s)
amadoriase
Systematic name
N-(1-deoxy-D-fructos-1-yl)amine:oxygen 2-oxidoreductase (glucosone-forming)
Comment
Reducing sugars such as glucose react with amino groups in proteins via the spontaeous Maillard reaction, forming an unstable product that undergoes spontaneous rearrangement to a keto amine compound. These reactions are known as glycation reactions, and the stable products are known as Amadori products. This enzyme, which contains an FAD cofactor, catalyses a deglycation reaction that regenerates the amine reactant. By-products are glucosone and hydrogen peroxide. The enzymes have been reported from fungi and bacteria, but not from higher eukaryotes. Specific enzymes differ in their substrate specificity. cf. EC 1.5.3.26, fructosyl amine oxidase (fructosamine-forming).
History
created 2022
back to topEC Tree
1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.5.3.3 created 1961, deleted 1972 1.5.3.8 created 1989, deleted 1992 1.5.3.9 created 1989, modified 1999, deleted 2002 1.97.1.5 created 2000 deleted 2001 1.97.1.6 created 2000 deleted 2001 1.97.1.7 created 2000, deleted 2001
