EC Explorer

[browse EC tree] - [search]

EC 4.1.99.19 Details

show Brenda entry

EC number

4.1.99.19

Accepted name

2-iminoacetate synthase

Reaction

L-tyrosine + S-adenosyl-L-methionine + NADPH = 2-iminoacetate + 4-methylphenol + 5′-deoxyadenosine + L-methionine + NADP⁺ + H⁺

Other name(s)

thiH (gene name)

Systematic name

L-tyrosine 4-methylphenol-lyase (2-iminoacetate-forming)

Comment

Binds a [4Fe-4S] cluster that is coordinated by 3 cysteines and an exchangeable S-adenosyl-L-methionine molecule. The first stage of catalysis is reduction of the S-adenosyl-L-methionine to produce methionine and a 5-deoxyadenosin-5-yl radical that is crucial for the conversion of the substrate. The reductant is assumed to be NADPH, which is provided by a flavoprotein:NADPH oxidoreductase system [4]. Part of the pathway for thiamine biosynthesis.

History

created 2011, modified 2014

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.2 With a cytochrome as acceptor

1.1.3 With oxygen as acceptor

1.1.4 With a disulfide as acceptor

1.1.5 With a quinone or similar compound as acceptor

1.1.7 With an iron-sulfur protein as acceptor

1.1.9 With a copper protein as acceptor

1.1.98 With other, known, physiological acceptors

1.1.98.1 alcohol dehydrogenase (azurin)

1.1.98.2 glucose-6-phosphate dehydrogenase (coenzyme-F₄₂₀)

1.1.98.3 decaprenylphospho-β-D-ribofuranose 2-dehydrogenase

1.1.98.4 F₄₂₀H₂:quinone oxidoreductase

1.1.98.5 secondary-alcohol dehydrogenase (coenzyme-F₄₂₀)

1.1.98.6 ribonucleoside-triphosphate reductase (formate)

1.1.98.7 serine-type anaerobic sulfatase-maturating enzyme

1.1.99 With unknown physiological acceptors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.10.1 With NAD⁺ or NADP⁺ as acceptor

1.10.2 With a cytochrome as acceptor

1.10.3 With oxygen as acceptor

1.10.5 With a quinone or related compound as acceptor

1.10.9 With a copper protein as acceptor

1.10.99 With unknown physiological acceptors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.15 Acting on superoxide as acceptor

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.21.1 With NAD⁺ or NADP⁺ as acceptor

1.21.3 With oxygen as acceptor

1.21.4 With a disulfide as acceptor

1.21.98 With other, known, physiological acceptors

1.21.99 With unknown physiological acceptors

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.97 Other oxidoreductases

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)

4.1 Carbon-carbon lyases

4.1.1 Carboxy-lyases

4.1.2 Aldehyde-lyases

4.1.3 Oxo-acid-lyases

4.1.99 Other carbon-carbon lyases

4.1.99.1 tryptophanase

4.1.99.2 tyrosine phenol-lyase

4.1.99.3 deoxyribodipyrimidine photo-lyase

4.1.99.4 created 1981, deleted 2002

4.1.99.5 aldehyde oxygenase (deformylating)

4.1.99.6 created 1989, deleted 2000

4.1.99.7 created 1992 as EC 2.5.1.40, transferred 1999 to EC 4.1.99.7, deleted 2000

4.1.99.8 created 2000, deleted 2000

4.1.99.9 created 2000, deleted 2000

4.1.99.10 created 2000, deleted 2000

4.1.99.11 benzylsuccinate synthase

4.1.99.12 3,4-dihydroxy-2-butanone-4-phosphate synthase

4.1.99.13 (6-4)DNA photolyase

4.1.99.14 spore photoproduct lyase

4.1.99.15 S-specific spore photoproduct lyase

4.1.99.16 geosmin synthase

4.1.99.17 phosphomethylpyrimidine synthase

4.1.99.18 cyclic pyranopterin phosphate synthase

4.1.99.19 2-iminoacetate synthase

4.1.99.20 3-amino-4-hydroxybenzoate synthase

4.1.99.21 (5-formylfuran-3-yl)methyl phosphate synthase

4.1.99.22 GTP 3′,8-cyclase

4.1.99.23 5-hydroxybenzimidazole synthase

4.1.99.24 L-tyrosine isonitrile synthase

4.1.99.25 L-tryptophan isonitrile synthase

4.1.99.26 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one synthase

4.2 Carbon-oxygen lyases

4.3 Carbon-nitrogen lyases

4.4 Carbon-sulfur lyases

4.5 Carbon-halide lyases

4.6 Phosphorus-oxygen lyases

4.7 carbon-phosphorus lyases

4.8 Nitrogen-oxygen lyases

4.98 ATP-independent chelatases

4.99 Other lyases

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)