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EC 4.1.99.13 Details

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EC number

4.1.99.13

Accepted name

(6-4)DNA photolyase

Reaction

(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA)

Other name(s)

DNA photolyase, H64PRH, NF-10, phr (6-4), PL-(6-4), OtCPF1, (6-4) PHR, At64PHR

Systematic name

(6-4) photoproduct pyrimidine-lyase

CAS registry number

37290-70-3

Comment

A flavoprotein (FAD). The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases. This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH^-) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases [2].

History

created 2009

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.2 With a cytochrome as acceptor

1.1.3 With oxygen as acceptor

1.1.4 With a disulfide as acceptor

1.1.5 With a quinone or similar compound as acceptor

1.1.7 With an iron-sulfur protein as acceptor

1.1.9 With a copper protein as acceptor

1.1.98 With other, known, physiological acceptors

1.1.98.1 alcohol dehydrogenase (azurin)

1.1.98.2 glucose-6-phosphate dehydrogenase (coenzyme-F₄₂₀)

1.1.98.3 decaprenylphospho-β-D-ribofuranose 2-dehydrogenase

1.1.98.4 F₄₂₀H₂:quinone oxidoreductase

1.1.98.5 secondary-alcohol dehydrogenase (coenzyme-F₄₂₀)

1.1.98.6 ribonucleoside-triphosphate reductase (formate)

1.1.98.7 serine-type anaerobic sulfatase-maturating enzyme

1.1.99 With unknown physiological acceptors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.10.1 With NAD⁺ or NADP⁺ as acceptor

1.10.2 With a cytochrome as acceptor

1.10.3 With oxygen as acceptor

1.10.5 With a quinone or related compound as acceptor

1.10.9 With a copper protein as acceptor

1.10.99 With unknown physiological acceptors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.15 Acting on superoxide as acceptor

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.21.1 With NAD⁺ or NADP⁺ as acceptor

1.21.3 With oxygen as acceptor

1.21.4 With a disulfide as acceptor

1.21.98 With other, known, physiological acceptors

1.21.99 With unknown physiological acceptors

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.97 Other oxidoreductases

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)

4.1 Carbon-carbon lyases

4.1.1 Carboxy-lyases

4.1.2 Aldehyde-lyases

4.1.3 Oxo-acid-lyases

4.1.99 Other carbon-carbon lyases

4.1.99.1 tryptophanase

4.1.99.2 tyrosine phenol-lyase

4.1.99.3 deoxyribodipyrimidine photo-lyase

4.1.99.4 created 1981, deleted 2002

4.1.99.5 aldehyde oxygenase (deformylating)

4.1.99.6 created 1989, deleted 2000

4.1.99.7 created 1992 as EC 2.5.1.40, transferred 1999 to EC 4.1.99.7, deleted 2000

4.1.99.8 created 2000, deleted 2000

4.1.99.9 created 2000, deleted 2000

4.1.99.10 created 2000, deleted 2000

4.1.99.11 benzylsuccinate synthase

4.1.99.12 3,4-dihydroxy-2-butanone-4-phosphate synthase

4.1.99.13 (6-4)DNA photolyase

4.1.99.14 spore photoproduct lyase

4.1.99.15 S-specific spore photoproduct lyase

4.1.99.16 geosmin synthase

4.1.99.17 phosphomethylpyrimidine synthase

4.1.99.18 cyclic pyranopterin phosphate synthase

4.1.99.19 2-iminoacetate synthase

4.1.99.20 3-amino-4-hydroxybenzoate synthase

4.1.99.21 (5-formylfuran-3-yl)methyl phosphate synthase

4.1.99.22 GTP 3′,8-cyclase

4.1.99.23 5-hydroxybenzimidazole synthase

4.1.99.24 L-tyrosine isonitrile synthase

4.1.99.25 L-tryptophan isonitrile synthase

4.1.99.26 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one synthase

4.2 Carbon-oxygen lyases

4.3 Carbon-nitrogen lyases

4.4 Carbon-sulfur lyases

4.5 Carbon-halide lyases

4.6 Phosphorus-oxygen lyases

4.7 carbon-phosphorus lyases

4.8 Nitrogen-oxygen lyases

4.98 ATP-independent chelatases

4.99 Other lyases

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Release 2023.1 (January 2023)