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7.4.2.4: chloroplast protein-transporting ATPase

This is an abbreviated version!
For detailed information about chloroplast protein-transporting ATPase, go to the full flat file.

Word Map on EC 7.4.2.4

Reaction

ATP
+
H2O
+
chloroplast-protein[side 1]
=
ADP
+
phosphate
+
chloroplast-protein[side 2]

Synonyms

AtcpSecA, ATP synthase complex, cpSecA, EC 3.6.3.52, hsp93, Hsp93-III, Hsp93-V, RbcS TP, Rubisco small subunit transit peptide, SEC translocase/integrase, Sec-type system, Sec1, SEC2, SEC2 translocase, SecA, SecA protein

ECTree

     7 Translocases
         7.4 Catalysing the translocation of amino acids and peptides
             7.4.2 Linked to the hydrolysis of a nucleoside triphosphate
                7.4.2.4 chloroplast protein-transporting ATPase

Engineering

Engineering on EC 7.4.2.4 - chloroplast protein-transporting ATPase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agy1
-
a loss-of-function mutant of cpSecA, an albino or glassy yellow mutant, identified when screening a T-DNA insertion mutant collection. The mutant can grow normally on dim light with an exogenous carbon supply, but is subject to photo-oxidative stress in the light. Chloroplast biogenesis is clearly comprised in the mutant, and the transcription of both nucleus- and chloroplast-encoded photosynthetic proteins is repressed, whereas the transcription of mitochondrion-encoded respiratory complexes is induced
C199A/C205A
-
ratio of MgATPase activity in reduced/oxidized state is 0.79, compared to 1.7 in wild-type
C89A
-
ratio of MgATPase activity in reduced/oxidized state is 1.39, compared to 1.7 in wild-type
M231L
-
ratio of MgATPase activity in reduced/oxidized state is 1.0, compared to 1.7 in wild-type
M23L/M279L/M282L
-
ratio of MgATPase activity in reduced/oxidized state is 0.99, compared to 1.7 in wild-type
M23L/M279L/M282L/C89A
-
ratio of MgATPase activity in reduced/oxidized state is 1.08, compared to 1.7 in wild-type
M279L/M282L
-
ratio of MgATPase activity in reduced/oxidized state is 0.96, compared to 1.7 in wild-type
additional information
-
gene disruption mutants of homologs atHSP93-V, atHSP93-III, mutant plants are much smaller and paler than wild-type plants, mutant chloroplasts contain less thylakoid membrane but are still able to import a variety of precursor proteins at reduced rate