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Literature summary for 7.4.2.4 extracted from
- Reactive oxygen species affect ATP hydrolysis by targeting a highly conserved amino acid cluster in the thylakoid ATP synthase gamma subunit (2012), Biochim. Biophys. Acta, 1817, 2038-2048.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C199A/C205A | ratio of MgATPase activity in reduced/oxidized state is 0.79, compared to 1.7 in wild-type | Spinacia oleracea |
| C89A | ratio of MgATPase activity in reduced/oxidized state is 1.39, compared to 1.7 in wild-type | Spinacia oleracea |
| M231L | ratio of MgATPase activity in reduced/oxidized state is 1.0, compared to 1.7 in wild-type | Spinacia oleracea |
| M23L/M279L/M282L | ratio of MgATPase activity in reduced/oxidized state is 0.99, compared to 1.7 in wild-type | Spinacia oleracea |
| M23L/M279L/M282L/C89A | ratio of MgATPase activity in reduced/oxidized state is 1.08, compared to 1.7 in wild-type | Spinacia oleracea |
| M279L/M282L | ratio of MgATPase activity in reduced/oxidized state is 0.96, compared to 1.7 in wild-type | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| reactive oxygen species | reactive oxygen species dramatically decrease ATP synthesis in situ and affect the coupling factor CF1 portion in vitro. A conserved cluster of three methionines and a cysteine on the chloroplast gamma subunit are oxidized by reactive oxygen species. These residues are exclusive catalytic targets for hydrogen peroxide and singlet oxygen, although additional unknown amino acid targets might be involved in the latter reaction. The cluster is tightly integrated in catalytic turnover since mutants vary in MgATPase rates, stimulation by sulfite and chloroplast-specific gamma subunit redox-modulation | Spinacia oleracea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| thylakoid | - |
Spinacia oleracea | 9579 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
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Release 2023.1 (January 2023)
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