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4.3.1.2: methylaspartate ammonia-lyase

This is an abbreviated version!
For detailed information about methylaspartate ammonia-lyase, go to the full flat file.

Word Map on EC 4.3.1.2

Reaction

L-threo-3-methylaspartate
=
mesaconate
 +
NH3

Synonyms

3-methyl-L-aspartic acid ammonia-lyase, 3-methylaspartase, 3-methylaspartate ammonia lyase, 3-methylaspartate ammonia-lyase, ammonia-lyase, methylaspartate, beta-methylaspartase, Ch-MAL, MAL, methylaspartate ammonia lyase, methylaspartate ammonia-lyase, threo-3-methyl-L-aspartate ammonia-lyase, threo-3-methylaspartase ammonia-lyase

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.1 Ammonia-lyases
                4.3.1.2 methylaspartate ammonia-lyase

Crystallization

Crystallization on EC 4.3.1.2 - methylaspartate ammonia-lyase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion, well solution consists of 100 mM potassium phosphate, pH 8.0, and 32% ammonium sulfate, crystals diffract to 1.3 A
-
crystallized from ammonium sulfate
-
once recrystallized
-
sitting-drop vapour-diffusion, 0.0045 ml protein sample and 0.0045 ml precipitant, crystals are obtained using 20-25% polyethylene glycol 6000, 100 mM sodium acetate, pH 7.0, 25 mM Tris-HCl as precipitant solution and 16-22% ethylene glycol as additive, crystals diffract to 2.0 A
-
sitting-drop vapur diffusion against a well solution of 20-25% polyethylene glycol 6000, 100 mM sodium acetate, pH 7.0, 25 mM Tris-HCl pH 7.0 as precipitating solution and 16-22% ethylene glycol as additive, crystals diffract to 1.9 A
-