BRENDA - Enzyme Database
show all sequences of 4.3.1.2

Purification and properties of crystalline 3-methylaspartase from two facultative anaerobes, Citrobacter sp. strain YG-0504 and Morganella morganii strain YG-0601

Kato, Y.; Asano, Y.; Biosci. Biotechnol. Biochem. 59, 93-99 (1995)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
-
Citrobacter freundii
-
Morganella morganii
Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
1 mM, complete
Citrobacter freundii
5,5'-dithiobis(2-nitrobenzoate)
1 mM 54%, loss of activity
Morganella morganii
8-hydroxyquinoline
5 mM, 44% loss of activity
Citrobacter freundii
8-hydroxyquinoline
5 mM, 88% loss of activity
Morganella morganii
Ag+
complete
Citrobacter freundii
Ag+
complete
Morganella morganii
Al3+
5 mM AlCl3, 95% loss of activity
Morganella morganii
Ca2+
-
Clostridium tetanomorphum
Ca2+
CaCl2 5 mM, 83% loss of activity
Citrobacter freundii
Ca2+
5 mM CaCl2, 73% loss of activity
Morganella morganii
Cd2+
1 mM CdCl2, complete inhibition
Morganella morganii
Co2+
1 mM CoCl2, 60% loss of activity
Morganella morganii
Cu2+
-
Clostridium tetanomorphum
Cu2+
1 mM CuSO4, 47% loss of activity
Citrobacter freundii
Cu2+
1 mM CuSO4, 73% loss of activity
Morganella morganii
Fe2+
5 mM FeSO4, 56% loss of activity
Citrobacter freundii
Fe2+
5 mM FeSO4, 89% loss of activity
Morganella morganii
Fe3+
1 mM, 75% loss of activity
Citrobacter freundii
Fe3+
1 mM, 69% loss of activity
Morganella morganii
Hg+
complete
Citrobacter freundii
Hg+
complete
Morganella morganii
Hg2+
complete
Citrobacter freundii
Hg2+
complete
Morganella morganii
iodoacetate
1 mM, 86% loss of activity
Morganella morganii
additional information
-
Citrobacter freundii
additional information
-
Morganella morganii
N-ethylmaleimide
-
Clostridium tetanomorphum
N-ethylmaleimide
complete
Citrobacter freundii
N-ethylmaleimide
complete
Morganella morganii
p-chloromercuribenzoate
complete
Citrobacter freundii
p-chloromercuribenzoate
complete
Morganella morganii
Pb2+
5 mM PbCl2, 88% loss of activity
Morganella morganii
Sn2+
1 mM SnCl2, 51% loss of activity
Morganella morganii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cs+
-
Citrobacter freundii
Cs+
-
Morganella morganii
Li+
-
Citrobacter freundii
Li+
-
Morganella morganii
Mg2+
-
Clostridium tetanomorphum
Mg2+
-
Citrobacter freundii
Mg2+
-
Morganella morganii
Mn2+
activates
Clostridium tetanomorphum
Mn2+
activates
Citrobacter freundii
Mn2+
activates
Morganella morganii
Na+
-
Citrobacter freundii
Na+
-
Morganella morganii
Ni2+
activates
Clostridium tetanomorphum
Ni2+
activates
Citrobacter freundii
Ni2+
activates
Morganella morganii
Rb+
-
Citrobacter freundii
Rb+
-
Morganella morganii
Zn2+
activates
Clostridium tetanomorphum
Zn2+
activates
Citrobacter freundii
Zn2+
activates
Morganella morganii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Citrobacter freundii
44000
-
2 * 44000, SDS-PAGE
Morganella morganii
70000
-
gel filtration
Morganella morganii
79000
-
gel filtration
Citrobacter freundii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-threo-3-methylaspartate
Citrobacter freundii
-
?
-
-
-
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
L-threo-3-methylaspartate
Morganella morganii
-
?
-
-
-
additional information
Clostridium tetanomorphum
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
?
-
-
-
additional information
Clostridium tetanomorphum NCIMB 11547
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Citrobacter freundii
-
Citrobacter sp. strain YG-0504
-
Clostridium tetanomorphum
-
NCIMB 11547
-
Clostridium tetanomorphum NCIMB 11547
-
NCIMB 11547
-
Morganella morganii
-
-
-
Morganella morganii YG-0601
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Citrobacter freundii
-
Morganella morganii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Citrobacter freundii
additional information
-
-
Clostridium tetanomorphum
additional information
-
-
Morganella morganii
162
-
-
Citrobacter freundii
296
-
-
Morganella morganii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2S,3S)-3-ethylaspartic acid
-
6060
Citrobacter freundii
?
-
-
-
-
(2S,3S)-3-ethylaspartic acid
-
6060
Clostridium tetanomorphum
?
-
-
-
-
(2S,3S)-3-ethylaspartic acid
-
6060
Morganella morganii
?
-
-
-
-
3-chloroaspartate
-
6060
Clostridium tetanomorphum
chlorofumarate + NH3
-
6060
Clostridium tetanomorphum
-
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Citrobacter freundii
chlorofumarate + NH3
-
6060
Citrobacter freundii
-
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Morganella morganii
chlorofumarate + NH3
-
6060
Morganella morganii
-
3-chloroaspartate
-
6060
Clostridium tetanomorphum NCIMB 11547
chlorofumarate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Morganella morganii YG-0601
chlorofumarate + NH3
-
6060
Morganella morganii YG-0601
-
? + NH4Cl
-
6060
Citrobacter freundii
?
-
-
-
-
? + NH4Cl
-
6060
Morganella morganii
?
-
-
-
-
ethylfumarate + NH3
-
6060
Citrobacter freundii
?
-
-
-
-
ethylfumarate + NH3
-
6060
Clostridium tetanomorphum
?
-
-
-
-
ethylfumarate + NH3
-
6060
Morganella morganii
?
-
-
-
-
ethylfumarate + NH3
-
6060
Morganella morganii YG-0601
?
-
-
-
-
L-threo-3-methylaspartate
r
6060
Citrobacter freundii
mesaconate + NH3
-
6060
Citrobacter freundii
-
L-threo-3-methylaspartate
r
6060
Clostridium tetanomorphum
mesaconate + NH3
-
6060
Clostridium tetanomorphum
-
L-threo-3-methylaspartate
r
6060
Morganella morganii
mesaconate + NH3
-
6060
Morganella morganii
-
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Citrobacter freundii
mesaconate + NH3
-
6060
Citrobacter freundii
-
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Clostridium tetanomorphum
mesaconate + NH3
-
6060
Clostridium tetanomorphum
-
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Morganella morganii
mesaconate + NH3
-
6060
Morganella morganii
-
L-threo-3-methylaspartate
r
6060
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
L-threo-3-methylaspartate
r
6060
Morganella morganii YG-0601
mesaconate + NH3
-
6060
Morganella morganii YG-0601
-
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Morganella morganii YG-0601
mesaconate + NH3
-
6060
Morganella morganii YG-0601
-
L-threo-3-methylaspartate
-
6060
Citrobacter freundii
?
-
-
-
-
L-threo-3-methylaspartate
-
6060
Clostridium tetanomorphum
?
-
-
-
-
L-threo-3-methylaspartate
-
6060
Morganella morganii
?
-
-
-
-
additional information
-
6060
Citrobacter freundii
?
-
-
-
-
additional information
-
6060
Clostridium tetanomorphum
?
-
-
-
-
additional information
-
6060
Morganella morganii
?
-
-
-
-
additional information
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
6060
Clostridium tetanomorphum
?
-
-
-
-
additional information
-
6060
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
additional information
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
6060
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
additional information
-
6060
Morganella morganii YG-0601
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
-
Clostridium tetanomorphum
dimer
2 * 40000, SDS-PAGE
Citrobacter freundii
dimer
2 * 44000, SDS-PAGE
Morganella morganii
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
50
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
55
-
-
Clostridium tetanomorphum
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
-
Citrobacter freundii
additional information
-
-
Morganella morganii
45
-
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity
Citrobacter freundii
45
-
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity
Morganella morganii
50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity
Citrobacter freundii
50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity
Morganella morganii
55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost
Citrobacter freundii
55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost
Morganella morganii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate
Morganella morganii
8.5
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate
Citrobacter freundii
9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
11
pH 6.0: 10% of maximal activity, pH 11.0: 40% of maximal activity in the amination reaction of mesaconate
Morganella morganii
7
11
pH 7.0: about 10% of maximal activity, pH 11.0: about 90% of maximal activity in the amination reaction of mesaconate; pH 7.0: about 25% of maximal activity, pH 11.0: about 5% of maximal activity, in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
7
11
pH 7.0: about 10% of maximal activity, pH 11.0: about 80% of maximal activity in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
-
Citrobacter freundii
additional information
-
-
Morganella morganii
5
10
stable between, pH 5.0: inactivated rapidly below
Citrobacter freundii
6
8.5
stable between, pH 5.0: inactivated rapidly below
Morganella morganii
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Citrobacter freundii
-
Morganella morganii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
1 mM, complete
Citrobacter freundii
5,5'-dithiobis(2-nitrobenzoate)
1 mM 54%, loss of activity
Morganella morganii
8-hydroxyquinoline
5 mM, 44% loss of activity
Citrobacter freundii
8-hydroxyquinoline
5 mM, 88% loss of activity
Morganella morganii
Ag+
complete
Citrobacter freundii
Ag+
complete
Morganella morganii
Al3+
5 mM AlCl3, 95% loss of activity
Morganella morganii
Ca2+
-
Clostridium tetanomorphum
Ca2+
CaCl2 5 mM, 83% loss of activity
Citrobacter freundii
Ca2+
5 mM CaCl2, 73% loss of activity
Morganella morganii
Cd2+
1 mM CdCl2, complete inhibition
Morganella morganii
Co2+
1 mM CoCl2, 60% loss of activity
Morganella morganii
Cu2+
-
Clostridium tetanomorphum
Cu2+
1 mM CuSO4, 47% loss of activity
Citrobacter freundii
Cu2+
1 mM CuSO4, 73% loss of activity
Morganella morganii
Fe2+
5 mM FeSO4, 56% loss of activity
Citrobacter freundii
Fe2+
5 mM FeSO4, 89% loss of activity
Morganella morganii
Fe3+
1 mM, 75% loss of activity
Citrobacter freundii
Fe3+
1 mM, 69% loss of activity
Morganella morganii
Hg+
complete
Citrobacter freundii
Hg+
complete
Morganella morganii
Hg2+
complete
Citrobacter freundii
Hg2+
complete
Morganella morganii
iodoacetate
1 mM, 86% loss of activity
Morganella morganii
additional information
-
Citrobacter freundii
additional information
-
Morganella morganii
N-ethylmaleimide
-
Clostridium tetanomorphum
N-ethylmaleimide
complete
Citrobacter freundii
N-ethylmaleimide
complete
Morganella morganii
p-chloromercuribenzoate
complete
Citrobacter freundii
p-chloromercuribenzoate
complete
Morganella morganii
Pb2+
5 mM PbCl2, 88% loss of activity
Morganella morganii
Sn2+
1 mM SnCl2, 51% loss of activity
Morganella morganii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cs+
-
Citrobacter freundii
Cs+
-
Morganella morganii
Li+
-
Citrobacter freundii
Li+
-
Morganella morganii
Mg2+
-
Clostridium tetanomorphum
Mg2+
-
Citrobacter freundii
Mg2+
-
Morganella morganii
Mn2+
activates
Clostridium tetanomorphum
Mn2+
activates
Citrobacter freundii
Mn2+
activates
Morganella morganii
Na+
-
Citrobacter freundii
Na+
-
Morganella morganii
Ni2+
activates
Clostridium tetanomorphum
Ni2+
activates
Citrobacter freundii
Ni2+
activates
Morganella morganii
Rb+
-
Citrobacter freundii
Rb+
-
Morganella morganii
Zn2+
activates
Clostridium tetanomorphum
Zn2+
activates
Citrobacter freundii
Zn2+
activates
Morganella morganii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Citrobacter freundii
44000
-
2 * 44000, SDS-PAGE
Morganella morganii
70000
-
gel filtration
Morganella morganii
79000
-
gel filtration
Citrobacter freundii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-threo-3-methylaspartate
Citrobacter freundii
-
?
-
-
-
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
L-threo-3-methylaspartate
Morganella morganii
-
?
-
-
-
additional information
Clostridium tetanomorphum
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
?
-
-
-
additional information
Clostridium tetanomorphum NCIMB 11547
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Citrobacter freundii
-
Morganella morganii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Citrobacter freundii
additional information
-
-
Clostridium tetanomorphum
additional information
-
-
Morganella morganii
162
-
-
Citrobacter freundii
296
-
-
Morganella morganii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2S,3S)-3-ethylaspartic acid
-
6060
Citrobacter freundii
?
-
-
-
-
(2S,3S)-3-ethylaspartic acid
-
6060
Clostridium tetanomorphum
?
-
-
-
-
(2S,3S)-3-ethylaspartic acid
-
6060
Morganella morganii
?
-
-
-
-
3-chloroaspartate
-
6060
Clostridium tetanomorphum
chlorofumarate + NH3
-
6060
Clostridium tetanomorphum
-
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Citrobacter freundii
chlorofumarate + NH3
-
6060
Citrobacter freundii
-
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Morganella morganii
chlorofumarate + NH3
-
6060
Morganella morganii
-
3-chloroaspartate
-
6060
Clostridium tetanomorphum NCIMB 11547
chlorofumarate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Morganella morganii YG-0601
chlorofumarate + NH3
-
6060
Morganella morganii YG-0601
-
? + NH4Cl
-
6060
Citrobacter freundii
?
-
-
-
-
? + NH4Cl
-
6060
Morganella morganii
?
-
-
-
-
ethylfumarate + NH3
-
6060
Citrobacter freundii
?
-
-
-
-
ethylfumarate + NH3
-
6060
Clostridium tetanomorphum
?
-
-
-
-
ethylfumarate + NH3
-
6060
Morganella morganii
?
-
-
-
-
ethylfumarate + NH3
-
6060
Morganella morganii YG-0601
?
-
-
-
-
L-threo-3-methylaspartate
r
6060
Citrobacter freundii
mesaconate + NH3
-
6060
Citrobacter freundii
-
L-threo-3-methylaspartate
r
6060
Clostridium tetanomorphum
mesaconate + NH3
-
6060
Clostridium tetanomorphum
-
L-threo-3-methylaspartate
r
6060
Morganella morganii
mesaconate + NH3
-
6060
Morganella morganii
-
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Citrobacter freundii
mesaconate + NH3
-
6060
Citrobacter freundii
-
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Clostridium tetanomorphum
mesaconate + NH3
-
6060
Clostridium tetanomorphum
-
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Morganella morganii
mesaconate + NH3
-
6060
Morganella morganii
-
L-threo-3-methylaspartate
r
6060
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
L-threo-3-methylaspartate
r
6060
Morganella morganii YG-0601
mesaconate + NH3
-
6060
Morganella morganii YG-0601
-
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Morganella morganii YG-0601
mesaconate + NH3
-
6060
Morganella morganii YG-0601
-
L-threo-3-methylaspartate
-
6060
Citrobacter freundii
?
-
-
-
-
L-threo-3-methylaspartate
-
6060
Clostridium tetanomorphum
?
-
-
-
-
L-threo-3-methylaspartate
-
6060
Morganella morganii
?
-
-
-
-
additional information
-
6060
Citrobacter freundii
?
-
-
-
-
additional information
-
6060
Clostridium tetanomorphum
?
-
-
-
-
additional information
-
6060
Morganella morganii
?
-
-
-
-
additional information
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
6060
Clostridium tetanomorphum
?
-
-
-
-
additional information
-
6060
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
additional information
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
6060
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
additional information
-
6060
Morganella morganii YG-0601
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
-
Clostridium tetanomorphum
dimer
2 * 40000, SDS-PAGE
Citrobacter freundii
dimer
2 * 44000, SDS-PAGE
Morganella morganii
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
50
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
55
-
-
Clostridium tetanomorphum
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
-
Citrobacter freundii
additional information
-
-
Morganella morganii
45
-
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity
Citrobacter freundii
45
-
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity
Morganella morganii
50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity
Citrobacter freundii
50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity
Morganella morganii
55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost
Citrobacter freundii
55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost
Morganella morganii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate
Morganella morganii
8.5
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate
Citrobacter freundii
9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
11
pH 6.0: 10% of maximal activity, pH 11.0: 40% of maximal activity in the amination reaction of mesaconate
Morganella morganii
7
11
pH 7.0: about 10% of maximal activity, pH 11.0: about 90% of maximal activity in the amination reaction of mesaconate; pH 7.0: about 25% of maximal activity, pH 11.0: about 5% of maximal activity, in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
7
11
pH 7.0: about 10% of maximal activity, pH 11.0: about 80% of maximal activity in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
-
Citrobacter freundii
additional information
-
-
Morganella morganii
5
10
stable between, pH 5.0: inactivated rapidly below
Citrobacter freundii
6
8.5
stable between, pH 5.0: inactivated rapidly below
Morganella morganii
Other publictions for EC 4.3.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747577
Abidin
Modular enzymatic cascade syn ...
Clostridium tetanomorphum
Chemistry
24
17434-17438
2018
-
1
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
729599
PuthanVeetil
-
Enantioselective Synthesis of ...
Clostridium tetanomorphum
ChemCatChem
5
1325-1327
2013
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
729745
Raj
The roles of active site resid ...
Clostridium tetanomorphum
FEBS open bio
3
285-290
2013
-
-
1
-
11
-
-
7
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
1
1
-
-
8
1
-
-
-
-
-
-
-
-
1
-
-
11
-
-
-
-
7
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
8
1
-
-
-
-
-
-
-
9
9
729057
Raj
Characterization of a thermost ...
Carboxydothermus hydrogenoformans
Appl. Microbiol. Biotechnol.
94
385-397
2012
-
-
1
-
-
-
-
2
-
2
1
-
-
5
-
-
1
-
-
-
-
-
11
1
2
1
-
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
2
1
-
-
-
-
1
-
-
-
-
11
1
1
-
1
2
1
-
-
-
-
-
-
-
2
2
730442
Raj
Engineering methylaspartate am ...
Clostridium tetanomorphum
Nat. Chem.
4
478-484
2012
-
-
1
-
2
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
35
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
35
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
714983
Ramezani
Glutamate racemization and cat ...
Fusobacterium varium
FEBS J.
278
2540-2551
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
703197
Raj
Alteration of the diastereosel ...
Clostridium tetanomorphum
ChemBioChem
10
2236-2245
2009
-
-
1
-
3
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
2
1
-
-
6
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
6
1
-
-
-
-
-
-
-
8
8
682798
Potrykus
Proteomic investigation of glu ...
Fusobacterium varium
Proteomics
7
1839-1853
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682889
Lee
Synthesis of mono- and di-deut ...
Escherichia coli
Tetrahedron
63
4663-4668
2007
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663816
Asano
-
Structure and Function of Amin ...
Citrobacter amalonaticus, Citrobacter amalonaticus YG-1002, Citrobacter freundii, Citrobacter freundii YG.0504, Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920, Morganella morganii, Morganella morganii YG-0601
Biocatal. Biotransform.
22
131-138
2004
-
-
1
1
-
-
-
3
-
6
6
-
-
23
-
-
-
-
-
-
-
-
6
3
4
1
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
6
6
-
-
-
-
-
-
-
-
-
6
3
1
-
-
-
2
-
-
-
-
-
-
-
-
-
652354
Asuncion
The structure of 3-methylaspar ...
Clostridium tetanomorphum
J. Biol. Chem.
277
8306-8311
2002
-
-
1
1
-
-
-
-
-
-
1
1
-
2
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653894
Levy
Insights into enzyme evolution ...
Citrobacter amalonaticus
Structure
10
105-113
2002
-
-
1
1
-
-
-
-
-
2
1
1
-
2
-
-
1
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649166
Asuncion
Overexpression, purification, ...
Clostridium tetanomorphum
Acta Crystallogr. Sect. D
57
731-733
2001
-
-
1
1
-
-
-
1
-
-
1
2
-
4
-
-
1
-
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
-
-
-
4
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
653208
Bearne
Purification and characterizat ...
Fusobacterium varium
Mol. Cell. Biochem.
221
117-126
2001
1
-
-
-
-
-
-
2
-
9
2
1
-
2
-
-
1
-
-
-
1
-
4
1
-
-
-
-
-
2
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
9
2
1
-
-
-
1
-
-
1
-
4
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
6064
Gani
The methylaspartase reaction p ...
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
Bioorg. Med. Chem.
7
977-990
1999
-
-
-
-
-
-
1
-
-
2
-
3
-
12
-
-
1
1
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
3
-
-
-
1
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650542
Pollard
Mechanism of 3-methylaspartase ...
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
Bioorg. Med. Chem.
7
949-975
1999
-
-
1
-
-
-
4
2
-
2
-
2
-
12
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
2
-
2
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6062
Kato
Cloning, nucleotide sequencing ...
Citrobacter amalonaticus, Citrobacter amalonaticus TPU 6323, Citrobacter amalonaticus YG-1002, Clostridium tetanomorphum, Clostridium tetanomorphum NCIMB 11547
Appl. Microbiol. Biotechnol.
50
468-474
1998
-
-
1
-
-
-
-
-
-
-
-
5
-
15
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5670
Kato
3-Methylaspartate ammonia-lyas ...
Citrobacter amalonaticus, Citrobacter amalonaticus YG-1002, Citrobacter freundii, Citrobacter freundii TPU 0011, Citrobacter freundii YG-0504, Citrobacter koseri, Citrobacter koseri JCM 1658, Citrobacter koseri JCM 1659, Clostridium tetanomorphum, Morganella morganii, Morganella morganii NCIMB 10466, Morganella morganii NCIMB 232, Morganella morganii NCIMB 9525, Morganella morganii YG-0601, Raoultella planticola, Raoultella planticola IAM 1133
Arch. Microbiol.
168
457-463
1997
2
5
-
-
-
-
-
-
-
-
5
17
-
27
7
-
5
6
-
-
6
-
51
6
-
-
-
-
-
-
-
-
-
-
-
-
2
5
-
-
-
-
-
-
-
-
-
-
-
5
17
-
7
-
5
-
-
6
-
51
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6061
Gulzar
-
Preparation of N-substituted a ...
Escherichia coli
J. Chem. Soc. Perkin Trans. I
1197
649-655
1997
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
22
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
22
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6059
Kato
3-Methylaspartate ammonia-lyas ...
Citrobacter amalonaticus, Citrobacter amalonaticus YG-1002, Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920, Clostridium tetanomorphum NCIMB 11547
Appl. Microbiol. Biotechnol.
43
901-907
1995
-
-
-
1
-
-
22
-
-
19
2
2
-
23
-
-
1
-
-
-
4
-
31
2
-
1
-
4
-
2
-
2
-
-
-
-
-
-
-
-
1
-
-
-
22
-
-
-
19
2
2
-
-
-
1
-
-
4
-
31
2
1
-
4
-
2
-
2
-
-
-
-
-
-
-
6060
Kato
Purification and properties of ...
Citrobacter freundii, Clostridium tetanomorphum, Clostridium tetanomorphum NCIMB 11547, Morganella morganii, Morganella morganii YG-0601
Biosci. Biotechnol. Biochem.
59
93-99
1995
-
-
-
2
-
-
33
-
-
20
4
5
-
15
-
-
2
-
-
-
5
-
34
3
-
3
-
8
-
4
3
4
-
-
-
-
-
-
-
-
2
-
-
-
33
-
-
-
20
4
5
-
-
-
2
-
-
5
-
34
3
3
-
8
-
4
3
4
-
-
-
-
-
-
-
6057
Goda
Cloning, sequencing, and expre ...
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
Biochemistry
31
10747-10756
1992
-
-
1
-
-
-
-
-
-
1
2
4
-
15
-
-
-
1
-
-
1
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
4
-
-
-
-
-
-
1
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6058
Botting
Mechanism of C-3 hydrogen exch ...
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
Biochemistry
31
1509-1520
1992
-
-
-
-
-
-
-
-
-
3
-
4
-
13
-
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
4
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
6055
Botting
Substrate specificity of the 3 ...
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
Biochemistry
27
2953-2955
1988
-
-
-
-
-
-
-
-
-
-
-
1
-
12
-
-
-
1
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6056
Botting
Primary deuterium isotope effe ...
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
Biochemistry
27
2956-2959
1988
-
-
-
-
-
-
-
-
-
-
-
1
-
12
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6054
Akhtar
-
Enantiospecific synthesis of 3 ...
Clostridium tetanomorphum
Tetrahedron
43
5899-5908
1987
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6066
Botting
-
Mechanism of the enzymic elimi ...
Bacterium cadaveris, Clostridium tetanomorphum
J. Chem. Soc. Chem. Commun.
1987
1371-1373
1987
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
11
-
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-
-
-
-
-
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-
-
-
-
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-
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-
-
-
-
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-
-
-
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-
-
2
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6067
Akhtar
-
Stereochemical course of the e ...
Bacterium cadaveris, Clostridium tetanomorphum
Tetrahedron Lett.
28
2413-2416
1987
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6053
Akhtar
-
Enzymic synthesis of 3-halogen ...
Clostridium tetanomorphum
J. Chem. Soc. Chem. Commun.
1986
1290-1291
1980
-
1
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5997
Hanson
-
The enzymic elimination of amm ...
Bacterium cadaveris, Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
7
75-166
1972
-
-
-
-
-
-
1
-
-
-
1
3
-
13
-
-
-
2
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
3
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6051
Hsiang
-
beta-methylaspartase from Clos ...
Bacterium cadaveris, Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
Methods Enzymol.
13
347-353
1969
-
-
-
1
-
-
1
-
-
3
1
2
-
13
-
-
1
-
-
-
1
1
17
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
3
1
2
-
-
-
1
-
-
1
1
17
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
6050
Bright
Divalent metal activation of b ...
Clostridium tetanomorphum
Biochemistry
6
1191-1203
1967
-
-
-
-
-
-
3
-
-
9
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
9
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6065
Winkler
New substrates for beta-methyl ...
Clostridium tetanomorphum
Biochim. Biophys. Acta
146
287-289
1967
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6049
Bright
-
The preparation of crystalline ...
Clostridium tetanomorphum
Biochim. Biophys. Acta
44
586-588
1960
-
-
-
1
-
-
-
-
-
5
-
1
-
1
-
-
1
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
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-