Crystallization (Comment) | Organism |
---|---|
- |
Citrobacter freundii |
- |
Morganella morganii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | 1 mM, complete | Citrobacter freundii | |
5,5'-dithiobis(2-nitrobenzoate) | 1 mM 54%, loss of activity | Morganella morganii | |
8-hydroxyquinoline | 5 mM, 44% loss of activity | Citrobacter freundii | |
8-hydroxyquinoline | 5 mM, 88% loss of activity | Morganella morganii | |
Ag+ | complete | Citrobacter freundii | |
Ag+ | complete | Morganella morganii | |
Al3+ | 5 mM AlCl3, 95% loss of activity | Morganella morganii | |
Ca2+ | CaCl2 5 mM, 83% loss of activity | Citrobacter freundii | |
Ca2+ | - |
Clostridium tetanomorphum | |
Ca2+ | 5 mM CaCl2, 73% loss of activity | Morganella morganii | |
Cd2+ | 1 mM CdCl2, complete inhibition | Morganella morganii | |
Co2+ | 1 mM CoCl2, 60% loss of activity | Morganella morganii | |
Cu2+ | 1 mM CuSO4, 47% loss of activity | Citrobacter freundii | |
Cu2+ | - |
Clostridium tetanomorphum | |
Cu2+ | 1 mM CuSO4, 73% loss of activity | Morganella morganii | |
Fe2+ | 5 mM FeSO4, 56% loss of activity | Citrobacter freundii | |
Fe2+ | 5 mM FeSO4, 89% loss of activity | Morganella morganii | |
Fe3+ | 1 mM, 75% loss of activity | Citrobacter freundii | |
Fe3+ | 1 mM, 69% loss of activity | Morganella morganii | |
Hg+ | complete | Citrobacter freundii | |
Hg+ | complete | Morganella morganii | |
Hg2+ | complete | Citrobacter freundii | |
Hg2+ | complete | Morganella morganii | |
iodoacetate | 1 mM, 86% loss of activity | Morganella morganii | |
additional information | - |
Citrobacter freundii | |
additional information | - |
Morganella morganii | |
N-ethylmaleimide | complete | Citrobacter freundii | |
N-ethylmaleimide | - |
Clostridium tetanomorphum | |
N-ethylmaleimide | complete | Morganella morganii | |
p-chloromercuribenzoate | complete | Citrobacter freundii | |
p-chloromercuribenzoate | complete | Morganella morganii | |
Pb2+ | 5 mM PbCl2, 88% loss of activity | Morganella morganii | |
Sn2+ | 1 mM SnCl2, 51% loss of activity | Morganella morganii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | - |
Citrobacter freundii | |
Cs+ | - |
Morganella morganii | |
Li+ | - |
Citrobacter freundii | |
Li+ | - |
Morganella morganii | |
Mg2+ | - |
Citrobacter freundii | |
Mg2+ | - |
Clostridium tetanomorphum | |
Mg2+ | - |
Morganella morganii | |
Mn2+ | activates | Citrobacter freundii | |
Mn2+ | activates | Clostridium tetanomorphum | |
Mn2+ | activates | Morganella morganii | |
Na+ | - |
Citrobacter freundii | |
Na+ | - |
Morganella morganii | |
Ni2+ | activates | Citrobacter freundii | |
Ni2+ | activates | Clostridium tetanomorphum | |
Ni2+ | activates | Morganella morganii | |
Rb+ | - |
Citrobacter freundii | |
Rb+ | - |
Morganella morganii | |
Zn2+ | activates | Citrobacter freundii | |
Zn2+ | activates | Clostridium tetanomorphum | |
Zn2+ | activates | Morganella morganii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Citrobacter freundii |
44000 | - |
2 * 44000, SDS-PAGE | Morganella morganii |
70000 | - |
gel filtration | Morganella morganii |
79000 | - |
gel filtration | Citrobacter freundii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threo-3-methylaspartate | Citrobacter freundii | - |
? | - |
? | |
L-threo-3-methylaspartate | Clostridium tetanomorphum | - |
? | - |
? | |
L-threo-3-methylaspartate | Morganella morganii | - |
? | - |
? | |
additional information | Clostridium tetanomorphum | enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes | ? | - |
? | |
additional information | Clostridium tetanomorphum NCIMB 11547 | enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
Citrobacter sp. strain YG-0504 | - |
Clostridium tetanomorphum | - |
NCIMB 11547 | - |
Clostridium tetanomorphum NCIMB 11547 | - |
NCIMB 11547 | - |
Morganella morganii | - |
- |
- |
Morganella morganii YG-0601 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Citrobacter freundii |
- |
Morganella morganii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Citrobacter freundii |
additional information | - |
- |
Clostridium tetanomorphum |
additional information | - |
- |
Morganella morganii |
162 | - |
- |
Citrobacter freundii |
296 | - |
- |
Morganella morganii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-3-ethylaspartic acid | - |
Citrobacter freundii | ? | - |
? | |
(2S,3S)-3-ethylaspartic acid | - |
Clostridium tetanomorphum | ? | - |
? | |
(2S,3S)-3-ethylaspartic acid | - |
Morganella morganii | ? | - |
? | |
3-chloroaspartate | - |
Clostridium tetanomorphum | chlorofumarate + NH3 | - |
? | |
3-chloroaspartate | (2R,3S)-3-chloroaspartic acid | Citrobacter freundii | chlorofumarate + NH3 | - |
? | |
3-chloroaspartate | (2R,3S)-3-chloroaspartic acid | Morganella morganii | chlorofumarate + NH3 | - |
? | |
3-chloroaspartate | - |
Clostridium tetanomorphum NCIMB 11547 | chlorofumarate + NH3 | - |
? | |
3-chloroaspartate | (2R,3S)-3-chloroaspartic acid | Morganella morganii YG-0601 | chlorofumarate + NH3 | - |
? | |
? + NH4Cl | - |
Citrobacter freundii | ? | - |
? | |
? + NH4Cl | - |
Morganella morganii | ? | - |
? | |
ethylfumarate + NH3 | - |
Citrobacter freundii | ? | - |
? | |
ethylfumarate + NH3 | - |
Clostridium tetanomorphum | ? | - |
? | |
ethylfumarate + NH3 | - |
Morganella morganii | ? | - |
? | |
ethylfumarate + NH3 | - |
Morganella morganii YG-0601 | ? | - |
? | |
L-threo-3-methylaspartate | r | Citrobacter freundii | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | r | Clostridium tetanomorphum | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | r | Morganella morganii | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter freundii | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Clostridium tetanomorphum | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Morganella morganii | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | r | Clostridium tetanomorphum NCIMB 11547 | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Clostridium tetanomorphum NCIMB 11547 | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | r | Morganella morganii YG-0601 | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Morganella morganii YG-0601 | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | - |
Citrobacter freundii | ? | - |
? | |
L-threo-3-methylaspartate | - |
Clostridium tetanomorphum | ? | - |
? | |
L-threo-3-methylaspartate | - |
Morganella morganii | ? | - |
? | |
additional information | - |
Citrobacter freundii | ? | - |
? | |
additional information | - |
Clostridium tetanomorphum | ? | - |
? | |
additional information | - |
Morganella morganii | ? | - |
? | |
additional information | enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes | Clostridium tetanomorphum | ? | - |
? | |
additional information | - |
Clostridium tetanomorphum NCIMB 11547 | ? | - |
? | |
additional information | enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes | Clostridium tetanomorphum NCIMB 11547 | ? | - |
? | |
additional information | - |
Morganella morganii YG-0601 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Clostridium tetanomorphum |
dimer | 2 * 44000, SDS-PAGE | Morganella morganii |
dimer | 2 * 40000, SDS-PAGE | Citrobacter freundii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
maximal activity in the deamination reaction of L-threo-3-methylaspartate | Morganella morganii |
50 | - |
maximal activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter freundii |
55 | - |
- |
Clostridium tetanomorphum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Citrobacter freundii |
additional information | - |
- |
Morganella morganii |
45 | - |
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity | Citrobacter freundii |
45 | - |
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity | Morganella morganii |
50 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity | Citrobacter freundii |
50 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity | Morganella morganii |
55 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost | Citrobacter freundii |
55 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost | Morganella morganii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate | Morganella morganii |
8.5 | - |
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate | Citrobacter freundii |
9.7 | - |
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate | Citrobacter freundii |
9.7 | - |
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate | Morganella morganii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | pH 6.0: 10% of maximal activity, pH 11.0: 40% of maximal activity in the amination reaction of mesaconate | Morganella morganii |
7 | 11 | pH 7.0: about 10% of maximal activity, pH 11.0: about 80% of maximal activity in the deamination reaction of L-threo-3-methylaspartate | Morganella morganii |
7 | 11 | pH 7.0: about 25% of maximal activity, pH 11.0: about 5% of maximal activity, in the deamination reaction of L-threo-3-methylaspartate | Citrobacter freundii |
7 | 11 | pH 7.0: about 10% of maximal activity, pH 11.0: about 90% of maximal activity in the amination reaction of mesaconate | Citrobacter freundii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Citrobacter freundii |
additional information | - |
- |
Morganella morganii |
5 | 10 | stable between, pH 5.0: inactivated rapidly below | Citrobacter freundii |
6 | 8.5 | stable between, pH 5.0: inactivated rapidly below | Morganella morganii |