Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.1.3.41: 4-nitrophenylphosphatase

This is an abbreviated version!
For detailed information about 4-nitrophenylphosphatase, go to the full flat file.

Word Map on EC 3.1.3.41

Reaction

4-nitrophenyl phosphate
+
H2O
=
4-nitrophenol
 +
phosphate

Synonyms

K+-p-nitrophenyl phosphatase, K+-phosphatase, K+pNPPase, p-nitrophenyl phosphatase, p-nitrophenylphosphatase, p-NPPase, para-nitrophenyl phosphatase, PHO13, pNPP, PNPPase, TpNPPase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.41 4-nitrophenylphosphatase

Inhibitors

Inhibitors on EC 3.1.3.41 - 4-nitrophenylphosphatase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
arsenate
-
competitive. 10 mM, 39% residual activity
choline chloride
-
synergistic with ouabain
Ethacrynic acid
-
synergistic with ouabain
ethane diamine
-
inhibits Mn2+ stimulation of pNPPase (as well as K+ and Mg2+ stimulation)
Fluorescein isothiocyanate
-
i.e. FITC, inhibits both the ATPase and 4-nitrophenyl phosphatase activities of the enzyme, fluorescence labeling, inhibition depends on pH
NaCl
-
synergistic with ouabain
o-vanadate
-
inhibits the 4-nitrophenyl phosphatase activity
orthovanadate
-
complete inhibition of 4-nitrophenyl phosphatase activity, synergistic with ouabain
Ouabain
-
80% inhibition of 4-nitrophenyl phosphatase activity, synergistic with other inhibitors, overview
phosphate
-
competitive. 10 mM, 58% residual activity
vanadate
-
10 mM, 63% residual activity
additional information
-
not inhibitory: EDTA, calcium, copper, cobalt, levamisole, and 4-hydroxymercuribenzoate
-