Literature summary for 3.1.3.41 extracted from

Mendonca, N.N.; Masui, D.C.; McNamara, J.C.; Leone, F.A.; Furriel, R.P.
Long-term exposure of the freshwater shrimp Macrobrachium olfersii to elevated salinity: effects on gill (Na+,K+)-ATPase alpha-subunit expression and K+-phosphatase activity (2007), Comp. Biochem. Physiol. A, 146, 534-543.

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Inhibitors

Inhibitors	Comment	Organism	Structure
ATP	competitive	Macrobrachium olfersii
choline chloride	synergistic with ouabain	Macrobrachium olfersii
Ethacrynic acid	synergistic with ouabain	Macrobrachium olfersii
NaCl	synergistic with ouabain	Macrobrachium olfersii
orthovanadate	complete inhibition of 4-nitrophenyl phosphatase activity, synergistic with ouabain	Macrobrachium olfersii
Ouabain	80% inhibition of 4-nitrophenyl phosphatase activity, synergistic with other inhibitors, overview	Macrobrachium olfersii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics in freshwater and 21% salinity, overview	Macrobrachium olfersii
1.31	-	4-nitrophenyl phosphate	pH 7.5, 25°C, 21% salinity	Macrobrachium olfersii
1.33	-	K+	pH 7.5, 25°C, 21% salinity	Macrobrachium olfersii
1.72	-	4-nitrophenyl phosphate	pH 7.5, 25°C, fresh water	Macrobrachium olfersii
2.09	-	Mg2+	pH 7.5, 25°C, 21% salinity	Macrobrachium olfersii
11.4	-	NH4+	pH 7.5, 25°C, 21% salinity	Macrobrachium olfersii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Macrobrachium olfersii	-	-
plasma membrane	-	Macrobrachium olfersii	5886	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	required, stimulates the 4-nitrophenyl phosphatase activity	Macrobrachium olfersii
Mg2+	required, stimulates the 4-nitrophenyl phosphatase activity	Macrobrachium olfersii
NH4+	stimulates 4-nitrophenyl phosphatase activity	Macrobrachium olfersii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	Macrobrachium olfersii	-	4-nitrophenol + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Macrobrachium olfersii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partially by microsome preperation	Macrobrachium olfersii

Source Tissue

Source Tissue	Comment	Organism	Textmining
gill	-	Macrobrachium olfersii	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
123.6	-	4-nitrophenyl phosphatase activity	Macrobrachium olfersii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Macrobrachium olfersii	4-nitrophenol + phosphate	-	?
additional information	the freshwater shrimp Na+/K+-ATPase shows 4-nitrophenyl phosphatase activity	Macrobrachium olfersii	?	-	?

Synonyms

Synonyms	Comment	Organism
K+-phosphatase	-	Macrobrachium olfersii
p-nitrophenylphosphatase	-	Macrobrachium olfersii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Macrobrachium olfersii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Macrobrachium olfersii

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.018	-	ATP	pH 7.5, 25°C	Macrobrachium olfersii
0.305	-	Ouabain	pH 7.5, 25°C, 21% salinity	Macrobrachium olfersii
0.762	-	Ouabain	pH 7.5, 25°C, fresh water	Macrobrachium olfersii

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Release 2023.1 (January 2023)