2.1.1.103: phosphoethanolamine N-methyltransferase
This is an abbreviated version!
For detailed information about phosphoethanolamine N-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.103
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2.1.1.103
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phosphatidylcholine
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phosphocholine
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choline
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phospholipid
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plasmodium
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falciparum
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malaria
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betaine
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s-adenosylhomocysteine
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plant-like
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osmoprotectant
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cdp-choline
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s-adenosylmethionine-dependent
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transmethylation
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medicine
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halophyte
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s-adenosyl-l-methionine-dependent
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nematicide
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amodiaquine
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drug development
- 2.1.1.103
- phosphatidylcholine
- phosphocholine
- choline
- phospholipid
- plasmodium
- falciparum
- malaria
- betaine
- s-adenosylhomocysteine
-
plant-like
-
osmoprotectant
- cdp-choline
-
s-adenosylmethionine-dependent
-
transmethylation
- medicine
-
halophyte
-
s-adenosyl-l-methionine-dependent
-
nematicide
- amodiaquine
- drug development
Reaction
Synonyms
AtNMT1, DPR2, More, NMT1, NMT2, PEAMT, PEAMT1, Pfpmt, phosphodimethylethanolamine methyltransferase, phosphoethanolamine methyltransferase, phosphoethanolamine N-methyltransferase, phosphoethanolamine-N-methyltransferases, phosphomethylethanolamine N-methyltransferase, PMEAMT, PMT, PMT-1, PMT1, PMT2, PVX_083045, S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferase, SoPEAMT
ECTree
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Purification
Purification on EC 2.1.1.103 - phosphoethanolamine N-methyltransferase
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HisTrap column chromatography
Ni2+-nitrilotriacetic acid-agarose resin column chromatography and Superdex-200 gel filtration
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nickel HisTrap column chromatography and Superdex 75PG gel filtration
nickel-affinity column chromatography and gel filtration
purification of the recombinant protein by affinity chromatography is found to be problematic and subject to losses in enzyme activity. The inclusion of DTT, BSA, and EDTA prevents the loss of enzyme activity during elution from a nickel ion matrix
recombinant His6-tagged PMT from Escherichia coli strain Rosetta II (DE3) by nickel affinity chromatography, the tag is cleaved off by thrombin, followed by benzamidine adsorption chromatography, and gel filtration
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recombinant wild-type and mutant enzymes from Escherichia coli by affinity chromatography
B3L8G9
nickel HisTrap column chromatography and Superdex 75PG gel filtration