Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
expressed in Saccharomyces cerevisiae strain CPBY19 (cho2, opi3 mutant) and in Escherichia coli BL21 (DE3) cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by 5 mM choline | Arabidopsis thaliana | |
phosphocholine | 47% activity in the presence of 1 mM, 17% activity in the presence of 5 mM | Arabidopsis thaliana | |
S-adenosyl-L-homocysteine | 73% activity in the presence of 0.01 mM, 18% activity in the presence of 0.2 mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
N,N-dimethylethanolamine phosphate | pH and temperature not specified in the publication | Arabidopsis thaliana | |
0.16 | - |
N-methylethanolamine phosphate | pH and temperature not specified in the publication | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q944H0 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Arabidopsis thaliana |
purification of the recombinant protein by affinity chromatography is found to be problematic and subject to losses in enzyme activity. The inclusion of DTT, BSA, and EDTA prevents the loss of enzyme activity during elution from a nickel ion matrix | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PMEAMT cannot use phosphoethanolamine as a substrate, but assays using phosphomethylethanolamine as a substrate result in both phosphodimethylethanolamine and phosphocholine as products. PMEAMT is inhibited by the reaction products PCho and S-adenosyl-L-homocysteine | Arabidopsis thaliana | ? | - |
? | |
additional information | the enzyme cannot use phosphoethanolamine as substrate | Arabidopsis thaliana | ? | - |
? | |
N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | choline phosphate + S-adenosyl-L-homocysteine | - |
? | |
N-methylethanolamine phosphate + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | N,N-dimethylethanolamine phosphate + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + 2-(methylamino)ethyl phosphate | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + 2-(dimethylamino)ethyl phosphate | - |
? | |
S-adenosyl-L-methionine + phosphodimethylethanolamine | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + phosphocholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphomethylethanolamine N-methyltransferase | - |
Arabidopsis thaliana |
PMEAMT | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | Arabidopsis pmeamt T-DNA mutant (atpmeamt) lacks transcripts associated with PMEAMT but has no overt phenotype. The leaf membrane phospholipid profiles show a greater content of PtdMEA as the 34:3 lipid molecular species in atpmeamt plants relative to wild type | Arabidopsis thaliana |
physiological function | enzyme can rescue a choline auxotroph of yeast | Arabidopsis thaliana |