Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.103 extracted from

  • BeGora, M.D.; Macleod, M.J.; McCarry, B.E.; Summers, P.S.; Weretilnyk, E.A.
    Identification of phosphomethylethanolamine N-methyltransferase from Arabidopsis and its role in choline and phospholipid metabolism (2010), J. Biol. Chem., 285, 29147-29155.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana
expressed in Saccharomyces cerevisiae strain CPBY19 (cho2, opi3 mutant) and in Escherichia coli BL21 (DE3) cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by 5 mM choline Arabidopsis thaliana
phosphocholine 47% activity in the presence of 1 mM, 17% activity in the presence of 5 mM Arabidopsis thaliana
S-adenosyl-L-homocysteine 73% activity in the presence of 0.01 mM, 18% activity in the presence of 0.2 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
N,N-dimethylethanolamine phosphate pH and temperature not specified in the publication Arabidopsis thaliana
0.16
-
N-methylethanolamine phosphate pH and temperature not specified in the publication Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Q944H0
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Arabidopsis thaliana
purification of the recombinant protein by affinity chromatography is found to be problematic and subject to losses in enzyme activity. The inclusion of DTT, BSA, and EDTA prevents the loss of enzyme activity during elution from a nickel ion matrix Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PMEAMT cannot use phosphoethanolamine as a substrate, but assays using phosphomethylethanolamine as a substrate result in both phosphodimethylethanolamine and phosphocholine as products. PMEAMT is inhibited by the reaction products PCho and S-adenosyl-L-homocysteine Arabidopsis thaliana ?
-
?
additional information the enzyme cannot use phosphoethanolamine as substrate Arabidopsis thaliana ?
-
?
N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine
-
Arabidopsis thaliana choline phosphate + S-adenosyl-L-homocysteine
-
?
N-methylethanolamine phosphate + S-adenosyl-L-methionine
-
Arabidopsis thaliana N,N-dimethylethanolamine phosphate + S-adenosyl-L-homocysteine
-
?
S-adenosyl-L-methionine + 2-(methylamino)ethyl phosphate
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + 2-(dimethylamino)ethyl phosphate
-
?
S-adenosyl-L-methionine + phosphodimethylethanolamine
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + phosphocholine
-
?

Synonyms

Synonyms Comment Organism
phosphomethylethanolamine N-methyltransferase
-
Arabidopsis thaliana
PMEAMT
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction Arabidopsis pmeamt T-DNA mutant (atpmeamt) lacks transcripts associated with PMEAMT but has no overt phenotype. The leaf membrane phospholipid profiles show a greater content of PtdMEA as the 34:3 lipid molecular species in atpmeamt plants relative to wild type Arabidopsis thaliana
physiological function enzyme can rescue a choline auxotroph of yeast Arabidopsis thaliana