1.14.13.33: 4-hydroxybenzoate 3-monooxygenase [NAD(P)H]
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For detailed information about 4-hydroxybenzoate 3-monooxygenase [NAD(P)H], go to the full flat file.
Reaction
Synonyms
4-HBA 3-hydroxylase, 4-HBA 3-monooxygenase, 4-hydroxybenzoate 3-hydroxylase, 4-hydroxybenzoate 3-monooxygenase (NAD(P)H), 4-hydroxybenzoate 3-monooxygenase (NAD(P)H2), 4-hydroxybenzoate-3-hydroxylase, 4HB 3-hydroxylase, NAD(P)H-dependent PHBHCn1, NADPH-dependent PHBH, oxygenase, 4-hydroxybenzoate 3-mono- (reduced nicotinamide adenine dinucleotide (phosphate)), p-hydroxybenzoate hydroxylase, para-hydroxybenzoate-3-hydroxylases, PHBH, PHBHCn1, PHBHRo, PHBHRo1CP, PHBHRr, PobA, PRAI, Reut_B5020, Xcc0356
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Engineering
Engineering on EC 1.14.13.33 - 4-hydroxybenzoate 3-monooxygenase [NAD(P)H]
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additional information
in Pseudomonas putida KT2440 strains engineered to convert lignin-related aromatic compounds to muconic acid (MA), PHBH activity is rate-limiting, as indicated by the accumulation of 4-HBA, which ultimately limits MA productivity. Replacement of PobA, the native Pseudomonas putida PHBH, with PraI, a PHBH from Paenibacillus sp. JJ-1b with a broader nicotinamide cofactor preference, can alleviate this bottleneck
additional information
in Pseudomonas putida KT2440 strains engineered to convert lignin-related aromatic compounds to muconic acid (MA), PHBH activity is rate-limiting, as indicated by the accumulation of 4-hydroxybenzoate (4-HBA), which ultimately limits MA productivity. Replacement of PobA, the native Pseudomonas putida PHBH, with PraI, a PHBH from Paenibacillus sp. JJ-1b with a broader nicotinamide cofactor preference, can alleviate this bottleneck. Comparison of three Pseudomonas putida strains engineered to produce MA from p-coumarate (pCA), showing that expression of praI leads to lower 4-HBA accumulation and decreased NADP+/NADPH ratios relative to strains harboring pobA, indicative of a relieved 4-HBA bottleneck due to increased NADPH availability. In bioreactor cultivations, a strain exclusively expressing praI achieved a titer of 40 g/l MA at 100% molar yield and a productivity of 0.5 g/l/h