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1.14.12.10: benzoate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about benzoate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.10

Reaction

benzoate
+
NADH
+
H+
+
O2
=
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate
+
NAD+

Synonyms

BDO, BenA, benABC, benzoate 1,2-dioxygenase, benzoate 1,2-dioxygenase oxygenase, benzoate 1,2-dioxygenase system, benzoate dioxygenase, benzoate hydroxylase, benzoic hydroxylase, BZDO, BZDO enzyme, EC 1.13.99.2, oxygenase, benzoate 1,2-di-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.10 benzoate 1,2-dioxygenase

Specific Activity

Specific Activity on EC 1.14.12.10 - benzoate 1,2-dioxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.36
-
benzoate oxygenase
5
-
pH 7, 25°C, with 3-methylbenzoate as substrate
790
-
benzoate reductase, reduction of K3Fe(CN)6
additional information
-
The recombinant bacteria shows a BZDO activity of 1.2 mmol benzoate per gram cell dry weight, while Pseudomonas putida KTSY01 shows a BZDO activity of only 0.09 mmol.